Results 241 to 250 of about 6,830 (257)

Biochemical characterization of the RNase II family of exoribonucleases from the human pathogens Salmonella typhimurium and Streptococcus pneumoniae.

Biochemistry, 2009
Maturation, turnover, and quality control of RNA are performed by many different classes of ribonucleases. Escherichia coli RNase II is the prototype of the RNase II family of ribonucleases, a ubiquitous family of hydrolytic, processive 3' --> 5 ...
S. Domingues, R. Matos, Filipa P. Reis, A. Fialho, Ana Barbas, C. Arraiano   +5 more
semanticscholar   +1 more source

Chapter 9 In Vitro Assays of 5′ to 3′‐Exoribonuclease Activity

2008
The major cytoplasmic 5' to 3'-exoribonuclease activity is carried out by the Xrn1 protein in eukaryotic cells. A number of different approaches can be used to study multifunctional Xrn1 protein activity in vitro. In this chapter, we concentrate on methods used in our laboratory to analyze Xrn1 5' to 3'-exoribonuclease activity.
Pellegrini, O., Mathy, N., Condon, C., Bénard, L.   +3 more
openaire   +4 more sources

Simultaneous isolation of cytoplasmic endoribonuclease and exoribonuclease of Trypanosoma brucei

Molecular and Biochemical Parasitology, 1985
An endoribonuclease and an exoribonuclease have been isolated simultaneously from the cytoplasm of Trypanosoma brucei by hydroxyapatite column chromatography. The endoribonuclease produced oligonucleotides from poly(adenylic acid) with 5'-phosphate and 3'-OH termini. The exoribonuclease produced only ribonucleoside 5'-phosphates from poly(adenylic acid)
George O. Gbenle, George O. Gbenle
openaire   +3 more sources

Structures and exoribonuclease activity fonctions in arenavirus and coronavirus.

Virologie (Montrouge, France), 2013
RNA viruses encode dedicated protein machinery required through the viral life cycle. Some enzymatic activities are generally associated with RNA viruses such as RNA- or DNA-dependent RNA polymerases, RNA helicases or proteases. Some viral enzyme activities are however unique to some viral families.
Isabelle Imbert, François Ferron, Etienne Decroly, Bruno Canard, Mickaël Bouvet   +4 more
openaire   +3 more sources

5′ → 3′-Exoribonuclease from Rabbit Reticulocytes

2001
Publisher Summary It has been demonstrated that 5′ → 3′-ribonucleases play an important role in the decay of mRNAs in yeast cells. A 5′ → 3′-ribonuclease from yeast cells named Xrn1p has been purified and cloned. Other 5′ → 3′-exoribonucleases have been partially purified from nuclei of mammalian cells.
openaire   +3 more sources

XRN1: A Major 5′ to 3′ Exoribonuclease in Eukaryotic Cells

2012
The degradation of RNA is a critical aspect of gene regulation. Correspondingly, ribonucleases exist within the cell to degrade RNA in specific cellular contexts. An important and conserved ribonuclease is called XRN1. This enzyme, an exoribonuclease, degrades RNA in a processive 5' to 3' direction.
Sarah Geisler, Jeff Coller
openaire   +3 more sources

Major 3'-5' Exoribonucleases in the Metabolism of Coding and Non-coding RNA.

Progress in Molecular Biology and Translational Science, 2018
Ricardo F Dos Santos, A. P. Quendera, Sofia Boavida, André F. Seixas, C. Arraiano, J. M. Andrade   +5 more
semanticscholar   +1 more source

Immunohistochemical localization of exoribonucleases (DIS3L2 and XRN1) in intranuclear inclusion body disease

Neuroscience Letters, 2018
F. Mori, K. Tanji, Y. Miki, Y. Toyoshima, H. Sasaki, Mari Yoshida, A. Kakita, H. Takahashi, K. Wakabayashi   +8 more
semanticscholar   +1 more source

Activity and roles of Arabidopsis thaliana XRN family exoribonucleases in noncoding RNA pathways

Journal of plant research, 2016
Yukio Kurihara
semanticscholar   +1 more source

Purification of eukaryotic exoribonucleases following heterologous expression in bacteria and analysis of their biochemical properties by in vitro enzymatic assays.

Methods in molecular biology, 2015
R. Tomecki, Karolina Drazkowska, A. Krawczyk, Katarzyna Kowalska, A. Dziembowski   +4 more
semanticscholar   +1 more source