Results 71 to 80 of about 11,712,389 (339)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Search for optimal parameters of wire electrical discharge machining of parts made from E110 zirconium alloy

Омский научный вестник
The article proposes a method for calculating rational parameters of wire electrical discharge machining of zirconium alloy E110 using a rotatable central composite design of the second order.
N. V. Bobkov, A. A. Fedorov, S. V. Petrochenko, N. S. Artemenko, R. V. Chulkov   +4 more
doaj   +1 more source

Plot size and replications number for triticale experiments [PDF]

Ciência Rural
: The hybridization between wheat and rye crops resulted in the triticale crop, which presents rusticity, versatility in animal and human food and possibility of use as a cover plant.
Marcos Toebe, Alberto Cargnelutti Filho, Anderson Chuquel Mello, Rafael Rodrigues de Souza, Franciéle dos Santos Soares, Lucas Santos da Silva, Alexandre Segatto   +6 more
doaj   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Software development for experiment planning [PDF]

, 2020
Досліджено планування експерименту для сумішей. Враховано особливості планування із попередньою трансформацією симплексної підобласті в силу накладених обмежень на вміст компонентів.
Куценко, С. І., Резанова, В. Г.   +1 more
core  

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

xrdPlanner: exploring area detector geometries for powder diffraction and total scattering experiments

Journal of Synchrotron Radiation
xrdPlanner is a software package designed to aid in the planning and preparation of powder X-ray diffraction and total scattering beam times at synchrotron facilities.
Lennard Krause, Frederik Holm Gjørup, Mads Ry Vogel Jørgensen   +2 more
doaj   +1 more source

Transboundary marine spatial planning: a reflexive marine governance experiment?

, 2017
Because seas and coastlines are shared between states, the formulation and implementation of marine spatial planning (MSP) should be transboundary by nature. The main argument of this paper is that MSP should be organized as a transboundary policy-making
J. V. van Tatenhove
semanticscholar   +1 more source

Trail Planning, Construction and Maintenance

, 1992
30 pagesThis publication is a supplement to Trail Planning, Construction, and Maintenance: A Bibliography, published in 1984 as University of Minnesota Agricultural Experiment Station Miscellaneous Publication no. 26.University of Minnesota. Agricultural
University of Minnesota. Agricultural Experiment Station
core  

Integrating Regional Development and Planning into “Spatial Planning” in Finland: The Untapped Potential of the Kainuu Experiment

, 2018
With Finland’s accession to the European Union in 1995, a regional level of administration responsible for regulation-based land-use planning and incentive-driven regional development policy was introduced. The administration of both policies on the same
Mattila, Hanna, Purkarthofer, Eva, Mattila, Hanna; id_orcid   +2 more
core   +1 more source