Results 221 to 230 of about 6,611,994 (284)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

An Experimental Design to Investigate Item Parameter Drift. [PDF]

open access: yesAppl Psychol Meas
Baldwin P   +5 more
europepmc   +1 more source

Sustainable agronomic strategies and experimental design for Stevia cultivation in the high mountains region of Veracruz, Mexico. [PDF]

open access: yesSci Rep
Gutérrez-González CI   +4 more
europepmc   +1 more source

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