Results 131 to 140 of about 823,915 (314)
Design and Analysis of Monte Carlo Experiments
monte carlo experiments;simulation models;mathematical analysis;sensitivity analysis;experimental ...
Kleijnen, J.P.C.
core
Environment and Natural Resources JournalThis research studied the performance of ozonation process combined with calcium peroxide (CaO2) as a catalyst for the removal of diclofenac (DCF) from synthetic wastewater.
Papitchaya Chookaew +2 more
doaj +1 more source
Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology [PDF]
Iran Biomed J, 2018 Agha Amiri S +5 more
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
An integrated methodology for the design of Ro-Ro passenger ships
, 2010 The present paper provides a brief introduction to the holistic approach to ship design, defines the generic ship design optimization problem and demonstrates its solution by use of advanced optimization ...
Zaraphonitis, George +3 more
core
A new methodology to robustify an experimental design
A robust experimental design is a desired object for practitioners when there is uncertainty about any of the assumptions necessary to compute the optimal design. For instance, when they use non-linear models, which requires having nominal values of the parameters. Several alternatives have been developed in the literature to obtain robust experimental
Muñoz del Río, Alba +2 more
openaire +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells
FEBS Open Bio, EarlyView.Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim +4 more
wiley +1 more source