Results 111 to 120 of about 17,090,036 (281)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology [PDF]

Iran Biomed J, 2018
Agha Amiri S, Zarei N, Enayati S, Azizi M, Khalaj V, Shahhosseini S.   +5 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Optimization of Diclofenac Treatment in Synthetic Wastewater using Catalytic Ozonation with Calcium Peroxide as Catalyst

Environment and Natural Resources Journal
This research studied the performance of ozonation process combined with calcium peroxide (CaO2) as a catalyst for the removal of diclofenac (DCF) from synthetic wastewater.
Papitchaya Chookaew, Apiradee Sukmilin, Chalor Jarusutthirak   +2 more
doaj   +1 more source

Experimental study of the multi-factor coupling effect of coal dust explosion intensity based on Box-Behnken response surface

Baozha yu Chongji
Using the Box-Behnken experimental design method, the influence of multi-factor coupling effects on the intensity of coal dust explosion during the transient explosion reaction process was studied. Forty-five sets of explosion tests were carried out in a
Hongze GUO, Xi CHEN, Hengyi YANG
doaj   +1 more source

A new methodology to robustify an experimental design

A robust experimental design is a desired object for practitioners when there is uncertainty about any of the assumptions necessary to compute the optimal design. For instance, when they use non-linear models, which requires having nominal values of the parameters. Several alternatives have been developed in the literature to obtain robust experimental
Muñoz del Río, Alba, Casero Alonso, Víctor, Amo Salas, Mariano   +2 more
openaire   +1 more source

Exploring experimental research: Methodologies, designs, and applications across disciplines

Cambodian Journal of Educational and Social Sciences (CJESS)
Experimental research is a primary scientific method used to elucidate cause-and-effect correlations between variables in diverse fields. Specifically, this paper outlines the essential characteristics of experimental research, encompassing variable manipulation, controlled settings, random assignment, and precise measurement procedures to enable ...
openaire   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source