Generation of orthotopic pancreatic tumors and ex vivo characterization of tumor-infiltrating T cell cytotoxicity. [PDF]
, 2019 In vivo models of pancreatic cancer provide invaluable tools for studying disease dynamics, immune infiltration and new therapeutic strategies. The orthotopic murine model can be performed on large cohorts of immunocompetent mice simultaneously, is ...
McNeish, Iain A +2 more
core +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Surface electromyographic biofeedback as a rehabilitation tool for patients with global brachial plexus injury receiving bionic reconstruction [PDF]
, 2019 In patients with global brachial plexus injury and lack of biological treatment alternatives, bionic reconstruction, including the elective amputation of the functionless hand and its replacement with a prosthesis, has recently been described.
Hruby, Laura A +2 more
core +1 more source
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
The Rothamsted Classical Experiments
, 1993 Part 1: The contribution of long-term experiments to agriculture and forestry 1: The importance of long-term experimentation, T R E Southwood FRS 2: The Rothamsted classical experiments, A E Johnston 3: The Sanborn Field Experiment, J R Brown 4: Long ...
Johnston, A. E.
core
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Effects of Size and Temperature‐Dependent Thermal Conductivity on the Cooling of Pyroclasts in Air
Geochemistry, Geophysics, Geosystems, 2018 The knowledge of the cooling time scales of pyroclasts, in conditions of free to forced convection, is of paramount importance in microtextural analysis, development of welded deposits, and in eruption column and pyroclast flow modeling.
Pranabendu Moitra +2 more
doaj +1 more source
Workshop: Experimenting with design experiments
, 2013 This (proposal for a) full day workshop intends to explore design experiments to create a deeper understanding of the underpinning mindsets, epistemological assumptions and their implications as well as possibilities within the context of academic ...
Westerlund, Bo, +3 more
core
Culturing In vivo-like murine astrocytes using the fast, simple, and inexpensive AWESAM protocol [PDF]
, 2017 The AWESAM (a low-cost easy stellate astrocyte method) protocol entails a fast, simple, and inexpensive way to generate large quantities of in vivo-like mouse and rat astrocyte monocultures: Brain cells can be isolated from different brain regions, and ...
Dean, Camin +3 more
core +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source