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Chapter 14 Explant Methods for Epidermal Cell Culture
1980Publisher Summary This chapter discusses the explant methods for epidermal cell culture. Full-thickness human skin is composed of epidermis, dermis, and subcutaneous tissue. Dermal components such as fibroblasts have been useful in studies on somatic cell genetics, cellular aging, and transformation.
Susan M. Fischer +3 more
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Effects of hyperthermia on cultured explants of porcine endometrium
British Veterinary Journal, 1986Abstract Explants of endometrium were cultured in medium M199 plus 10% sow's serum, and in a gas phase of 5% CO2 in air, for up to 13 days at temperatures of either 38 °C or 41 °C. Assessed by gross and microscopical appearance, survival of the explants was significantly worse at 41 °C.
M. Trujano, A.E. Wrathall
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Cytologic and hormonal activity of trophoblast in explant culture
American Journal of Obstetrics and Gynecology, 1966Abstract Trophoblastic tissue from 13 ectopic pregnancies and 2 hydatidiform moles have been grown in prolonged explant cultures. Quantitative gonadotropin hormone production and characteristics of cell growth are described. From these studies, it is observed that a characteristic cell, which migrates from the explant and displays a broad and ...
Thomas C. Smith +3 more
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Cleavage Blastomere Explant Culture in Xenopus
Cold Spring Harbor Protocols, 2018The individual blastomeres of Xenopus two- to 32-cell embryos have been fate mapped. This work identified the precursors of most of the embryonic cell types, tissues and organs; however, the maps do not reveal the cell interactions or signaling pathways that are required for establishing cell fates.
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Embryo Culture in Explanted Oviducts in Mice and Cattle
Hormone Research, 1995Developmental block of early embryos is due to the lack of some components in the culture medium and/or caused by inappropriate environmental conditions for embryonic development. It was shown in our experiments that the development of mouse and bovine embryos under the influence of oviducts in vitro can provide us with a model to analyze the oviductal
Yoshihiko Hosoi +2 more
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In vitro culture of equine respiratory mucosa explants
The Veterinary Journal, 2009An in vitro model of the upper respiratory tract of the horse was developed to investigate mechanisms of respiratory diseases. Four tissues of the upper respiratory tract of three horses were collected. Explants were maintained in culture at an air-liquid interface for 96h.
Sarah Glorieux +5 more
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Manipulation of Shoot Formation in Cultured Explants
2003Regeneration of buds, shoots, and roots in cells and explants in vitro has provided useful developmental systems to analyze the processes of cell differentiation and morphogenesis. Interest in these studies was greatly stimulated by the demonstration of chemical regulation of morphogenesis in 1957 by Skoog and Miller (1).
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Ciliary epithelia of the mammalian eye in cultured explants
Cell and Tissue Research, 1983The ultrastructural characteristics of ciliary epithelium from bovine, pigmented rabbit, and fetal albino rabbit were studied in cultured explants. The tips of ciliary processes were cultured in plastic dishes with Dulbecco Modified Eagle Medium (DMEM) containing 5% fetal bovine serum. More than half of the explants adhered to the plastic culture dish,
Miguel Coca-Prados +2 more
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Cell Proliferation in Explant Cultures of Human Colon
Digestion, 1982Biopsy specimens of human colonic mucosa taken from the rectosigmoid of 12 normal subjects were maintained in explant culture for 4 days. Histological, microautoradiographic and chemical measurements were carried out to evaluate cell replication, the effect of deoxycholic acid, and the incorporation of uridine and leucine into RNA and protein.
L. Augenlicht +5 more
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Culturing spinal cord explants in a collagen gel
Journal of Neuroscience Methods, 1984A new method for culturing spinal cord slices or explants is presented which entails the use of a commercially available purified collagen, Vitrogen. Vitrogen provides a stable three-dimensional matrix for culturing spinal cord explants which is superior to the conventional method of applying explants to moist dishes coated with rat tail collagen.
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