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Manipulation of Shoot Formation in Cultured Explants

2003
Regeneration of buds, shoots, and roots in cells and explants in vitro has provided useful developmental systems to analyze the processes of cell differentiation and morphogenesis. Interest in these studies was greatly stimulated by the demonstration of chemical regulation of morphogenesis in 1957 by Skoog and Miller (1).
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Phagocytosis by retinal pigment epithelium explants in culture

Experimental Eye Research, 1981
Phagocytosis of rod outer segments and of latex beads was demonstrated in retinal pigment epithelium explants from adult albino rats. Uptake of latex particles increased steadily over a 24 hr period while ingestion of rod outer segments peaked after 2 hr of incubation, then decreased.
N J, Philp, M H, Bernstein
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Cell Proliferation in Explant Cultures of Human Colon

Digestion, 1982
Biopsy specimens of human colonic mucosa taken from the rectosigmoid of 12 normal subjects were maintained in explant culture for 4 days. Histological, microautoradiographic and chemical measurements were carried out to evaluate cell replication, the effect of deoxycholic acid, and the incorporation of uridine and leucine into RNA and protein.
M, Usugane   +5 more
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Limbal explant cultures on amniotic membrane: The effects of passaging the explants on cell phenotype

Experimental Eye Research
In vitro expansion of limbal epithelial stem cells (LESCs) while maintaining their characteristics has the potential to address the urgent need in ophthalmology clinics for the treatment of limbal stem cell deficiency (LSCD). Herein, we investigated the impact of explant passaging on the phenotype of LESCs cultured on human amniotic membrane (hAM ...
Mehmet Gurdal   +3 more
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Cytologic and hormonal activity of trophoblast in explant culture

American Journal of Obstetrics and Gynecology, 1966
Abstract Trophoblastic tissue from 13 ectopic pregnancies and 2 hydatidiform moles have been grown in prolonged explant cultures. Quantitative gonadotropin hormone production and characteristics of cell growth are described. From these studies, it is observed that a characteristic cell, which migrates from the explant and displays a broad and ...
R A, Pattillo   +3 more
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Cleavage Blastomere Explant Culture in Xenopus

Cold Spring Harbor Protocols, 2018
The individual blastomeres of Xenopus two- to 32-cell embryos have been fate mapped. This work identified the precursors of most of the embryonic cell types, tissues and organs; however, the maps do not reveal the cell interactions or signaling pathways that are required for establishing cell fates.
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Embryo Culture in Explanted Oviducts in Mice and Cattle

Hormone Research, 1995
Developmental block of early embryos is due to the lack of some components in the culture medium and/or caused by inappropriate environmental conditions for embryonic development. It was shown in our experiments that the development of mouse and bovine embryos under the influence of oviducts in vitro can provide us with a model to analyze the oviductal
Y, Hosoi, N, Minami, A, Iritani
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Human placental explants in culture: Approaches and assessments

Placenta, 2005
Placental explant cultures in vitro are useful for studying tissue functions including cellular uptake, production and release of secretory components, cell interactions, proliferation, growth and differentiation, gene delivery, pharmacology, toxicology, and disease processes.
R K, Miller   +5 more
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Biosynthesis of IgG in periapical lesion explant cultures

Journal of Endodontics, 1991
The presence of immunoglobulins has been demonstrated in periapical inflammatory lesions associated with endodontic disease. The purpose of this study was to determine if IgG is synthesized in vitro in explant cultures of untreated periapical inflammatory lesions and to determine the level of IgG in isolated samples.
J C, Baumgartner, W A, Falkler
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Growth and differentiation of urothelial cells in explant culture

Pflügers Archiv European Journal of Physiology, 1996
Growth and differentiation of urothelial cells in explant cultures of mouse urinary bladder were studied by means of scanning and transmission electron microscopy and lectin binding analysis. The proliferation and growth of epithelial cells over the cut edges of the explant and over the substrate began after explant attachment to the substrate was ...
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