Results 181 to 190 of about 251,489 (270)

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

The Interaction between Sleep and Development on Wake EEG Oscillations. [PDF]

eNeuro
Snipes S, Jaramillo V, Krugliakova E, Volk C, Furrer M, Studler M, LeBourgeois M, Kurth S, Jenni OG, Huber R.   +9 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

AreTomoLive: automated reconstruction of comprehensively corrected and denoised cryo-electron tomograms in real time and at high throughput. [PDF]

Nat Methods
Peck A, Yu Y, Paraan M, Kimanius D, Ermel UH, Hutchings J, Schwartz J, Serwas D, Siems H, Hill NS, Ali M, Peukes J, Greenan GA, Sheu SH, Montabana EA, Carragher B, Potter CS, Agard DA, Zheng S.   +18 more
europepmc   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Individualizing beta-lactam dosing in real-world patients: lessons from a pharmacist-led programme implementation and evaluation. [PDF]

JAC Antimicrob Resist
Pan S, Weslander E, Fierek S, Ball M, Crutchfield CA, Galvin S, Rhodes NJ.   +6 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Evidence of decreased neural efficiency supporting fluid reasoning abilities in children and adolescents chronically exposed to home radon. [PDF]

Neurotoxicol Teratol
Greenwood SL, Pulliam HR, Clarke-Smith MN, Rasmussen SC, Parolek GE, Smith OV, Taylor BK.   +6 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Sleep-dependent infraslow rhythms are evolutionarily conserved across reptiles and mammals. [PDF]

Nat Neurosci
Bergel A, Schmidt JM, Barrillot B, Arthaud S, Averty L, Blumberg MS, Carachet C, Clair A, Filchenko I, Froidevaux C, Herrel A, Massot B, Rattenborg NC, Schmidt MH, Tanter M, Ungurean G, Libourel PA.   +16 more
europepmc   +1 more source