Results 191 to 200 of about 1,592,514 (298)

Stage-specific threats reveal the inadequacy of adult-centered conservation. [PDF]

Elife
Song YF, Wang YL, Li QQ, Yuan ZY, Zhou WW.   +4 more
europepmc   +1 more source

THE EXTINCTION COEFFICIENTS OF THE REDUCED BAND OF PYRIDINE NUCLEOTIDES

Journal of Biological Chemistry, 1948
B L, HORECKER, A, KORNBERG
openaire   +2 more sources

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

Next‐Generation Glutamine Synthetase Expression Vector for Therapeutic Protein Manufacturing Using CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari, Sophie J. Wensley, Malak A. Ammar, Avinash Srivastava, Changhavi Ragurajan, Jenish Patel, Natalie C. Elliott, Alice C. Hodgson‐Casson, Mariano Olivera Fedi, Michael Anbar, Peter M. O'Callaghan, Bernie Sweeney   +11 more
wiley   +1 more source

Study on the Photothermal Performance Enhancement of Direct Absorption Solar Collectors by Heat-Transfer-Oil-Based MWCNT-TiN Composite Nanofluids. [PDF]

ACS Omega
Hu J, Gong D, Yan J, Peng C, Gan G, Xie Y, Jian Z.   +6 more
europepmc   +1 more source

Nuclease‐Free Scalable Purification of Physalis Mottle Virus‐Like Particles Expressed in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Plant virus‐like particles (VLPs) are promising nanotechnology platforms, but their translation requires purification strategies suitable for large‐scale production. Here, we developed a nuclease‐free downstream process for Physalis mottle virus (PhMV) VLPs expressed in Escherichia coli, combining ammonium sulfate precipitation, low‐pH ...
Sean K. Hsu, Patrick Opdensteinen, Tariq Hussain, Nicole F. Steinmetz   +3 more
wiley   +1 more source

MPS Content-Dependent Tuning of Optical Properties of MPS-Capped CdS Quantum Dot Thin Films. [PDF]

Nanomaterials (Basel)
Koç K, Kar A, Okutan M.
europepmc   +1 more source

Feasibility Assessment of a Single‐pH Connected Polishing Train for Monoclonal Antibody Purification Using Mildly Acidic Protein A Elution and an Acidic‐Window Anion Exchange Membrane

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody downstream processing often requires multiple pH adjustments between Protein A capture and polishing steps. These adjustments increase process complexity and make direct unit‐operation connection more difficult. We evaluated a connected post‐capture polishing block operated at approximately pH 5.0 using mild‐pH Protein A ...
Takaki Aiso, Hiroshi Muto, Kyota Suzuki, Yumiko Masuda, Hirofumi Kakihara, Masashi Sonoyama   +5 more
wiley   +1 more source

Tailoring the Structural, Optical, and Electrical Performance of PVA Through Sn_0.8Mn_0.2S Nanostructures Incorporation for Optoelectronic Applications. [PDF]

Polymers (Basel)
Althobaiti MG.
europepmc   +1 more source

Digital cover photography for estimating leaf area index (LAI) in apple trees using a variable light extinction coefficient. [PDF]

Sensors (Basel), 2015
Poblete-Echeverría C, Fuentes S, Ortega-Farias S, Gonzalez-Talice J, Yuri JA.   +4 more
europepmc   +1 more source