Results 121 to 130 of about 8,496,804 (298)

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

open access: yesFEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks   +4 more
wiley   +1 more source

Fluorescence based extracellular enzyme activity assay for early detection of biofouling in seawater desalination systems

open access: yes, 2017
A method and system for early detection of biofouling utilizing a fluorescence based extracellular enzyme activity assay is disclosed. The method provides a means for early detection of biofouling on membranes used in flow-through membrane filtration ...
Khan, Babar Khalid, Leiknes, TorOve
core  

Protease activity of extracellular enzyme produced by B. subtilis isolated from soil [PDF]

open access: yes, 2017
Background: Proteases produced by enzymatic method are more environments friendly than chemical process, and they have tremendous potential in the leather industry and in other several industries.
uddin, M. E. (Md)   +10 more
core  

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

open access: yesFEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph   +7 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

open access: yesFEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Enhancing Multi-Enzyme Cascade Activity in Metal-Organic Frameworks via Controlled Enzyme Encapsulation [PDF]

open access: yes
To position multi‐enzymes in a core‐shell structure, the conventional layer‐by‐layer approach is often used. However, this method is time‐consuming and complex, requiring multiple steps and the isolation of intermediates at each stage.
Liang, Kang   +4 more
core   +1 more source

Analysis of Extracellular Enzyme Activity of Microbial Communities in Wetland Soil [PDF]

open access: yes
Wetlands hold relatively large amounts of carbon compared to other environmental carbon storages. Studying carbon degradation pathways of wetland soil microbial communities is important in understanding nutrient cycling.
Becket, Elinne   +4 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

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