Results 91 to 100 of about 1,984,454 (288)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Development and validation of a bioanalytical hplc-dad method for ketoprofen quantification in rat plasma and brain tissue: application to pharmacokinetic studies and tissue distribution analysis

Bioscience Journal
Ketoprofen (KTP) is used as an anti-inflammatory agent and has been studied as a potential treatment for neurodegenerative diseases. This study developed and validated a sensitive high-performance liquid chromatography with diode-array detection method ...
Camila Oliveira Pacheco, Jamily Rosa Langendorf , Joane Guterres Ferreira , Pedro Santos da Silva , Tamara Ramos Maciel, Sandra Elisa Haas   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Reliability of Extraction/Chromatography RIAs

Clinical Chemistry, 2004
In their editorial (1) in a recent issue of Clinical Chemistry , entitled “Immunoassays for Testosterone in Women: Better than a Guess?”, David Herold and Robert Fitzgerald discussed the article by Taieb et al. (2) that appeared in the same issue. The latter authors compared serum testosterone concentrations measured with 10 different commercially ...
openaire   +1 more source

A North Cameroonian cultivar of Hibiscus sabdariffa (Malvaceae) with calyces enriched in anthocyanins

International Journal of Plant Based Pharmaceuticals
Folere (Hibiscus sabdariffa L.) is a traditional plant cultivated in the north of Cameroon near the city of Yagoua. Calyces are used to prepare beverages while the leaves are incorporated into meals. This Cameroonian roselle is characterized by a higher
Raynatou Ba Ibrahim, Sarah Orion, Olaf Werner, Pauline Gautier, Brigitte Njikeuntchi–Bureau, Loic Bureau, Brice Autier, Marylène Chollet-Krugler, Françoise Lohézic–Le Dévéhat   +8 more
doaj   +1 more source

Urinary 5α-Androstanediol and 5β-Androstanediol Measurement by Gas Chromatography after Solid-Phase Extraction and High-Performance Liquid Chromatography

The International Journal of Biological Markers, 1999
Urinary androstanediol measurement, often in association with other androgens, is commonly used to support the clinical diagnosis of idiopathic hirsutism.
G.J. Minut, M. CambiÈ, M. Lanzoni, E. Marubini, G. Secreto   +4 more
doaj   +1 more source

Separation of cyclotron-produced cobalt-55/58m from iron targets using cation exchange chromatography with non-aqueous solvents and extraction chromatography. [PDF]

Appl Radiat Isot, 2023
Lin W, Aluicio-Sarduy E, Barrett KE, Barnhart TE, Mixdorf JC, DeLuca MC, Engle JW.   +6 more
europepmc   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study

Green Analytical Chemistry
Chebulagic acid (CA), a hydrolysable tannin found in Terminalia bellirica (T. bellirica) (Gaertn.) Roxb., has gained prominence as a significant component in Ayurvedic medicine. This study optimized methods for determining CA in T. bellirica, focusing on
Nisha Nerlekar, Aditya Magdum, Vinod Nandre, Mansingraj Nimbalkar, Padma Dandge   +4 more
doaj   +1 more source

High-Throughput Determination of Ultratrace Actinides in Urine by In-Line Extraction Chromatography Combined with Quadrupole Inductively Coupled Plasma Mass Spectrometry (EC-ICP-MS). [PDF]

Anal Chem, 2022
Liu Y, Xiao G, Jones RL.
europepmc   +1 more source