Results 81 to 90 of about 1,984,454 (288)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

New Technologies in Manufacture of Original Medical Preparations

Eurasian Chemico-Technological Journal, 2018
The progressive, ecologically pure technologies of isolation of sesquiterpene €-lactones of plant originwere examined in this article. The original phytopreparations were developed on their basis. The pharmacoeconomic characteristics of the obtaining of
Ye. G. Tolokonnikov
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Advanced sample preparation and chromatographic techniques for analyzing plant-based bioactive chemicals in nutraceuticals

Journal of Chromatography Open
Since ancient times, humanity has used plants to cure diseases or maintain health. Nowadays, obtaining the bioactive components of plants and researching their effects continues at great speed.
M. Mesud Hurkul, Ahmet Cetinkaya, Seyda Yayla, Sibel A. Ozkan   +3 more
doaj   +1 more source

Liquid chromatography tandem mass spectrometry for determination of catecholamines using alumina-based extraction method

, 2007
Catecholamines including epinephrine, norepinephrine and dopamine are important neurotransmitters in human species. The levels of catecholamines in biologic sample are utilized to diagnose hypertension, pheochromocytoma and neuroblastoma.
石先哲, 许国旺, 顾群, 路鑫
core  

Comparison of different methods for extraction from Tetraclinis articulata: Yield, chemical composition and antioxidant activity [PDF]

, 2013
In the present study, three techniques of extraction: hydrodistillation (HD), solvent extraction (conventional ‘Soxhlet’ technique) and an innovative technique, i.e., the supercritical fluid extraction (SFE), were applied to ground Tetraclinis articulata
Romdhane, Mehrez, Herzi, Nejia, Bouajila, Jalloul, Camy, Séverine, Condoret, Jean-Stéphane   +4 more
core   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

FEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito, Haduki Asano, Ayano Ueki, Joe Sakamoto, Yasuhiro Kamei, Hayato Tokumoto, Ayaka Yazawa, Shigeki Kamitani   +7 more
wiley   +1 more source

Extraction of trans-Anethole from Star Anise (Illicium verum) Using Combination of Microwave, Ultrasonic, and Enzyme Assisted Methods and Evaluation of Their Antibacterial Activity

Indonesian Journal of Chemistry
This study examines the efficiency of non-conventional extraction methods to obtain trans-anethole from Illicium verum using single and combination extraction techniques: microwave assisted extraction (MAE), ultrasound-assisted extraction (UAE), and ...
Elvina Dhiaul Iftitah, Warsito Warsito, Vivi Nurhadianty, Fitri Ariadna Sodi Miranda, Rafika Nur Hafidah   +4 more
doaj   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source