Results 41 to 50 of about 697,801 (350)

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Risk Factors and Effectiveness of Implementing Multi-Construction Projects for Development of Industrial Facility Sector in Indonesia

IJEBD (International Journal of Entrepreneurship and Business Development)
Purpose: Multi-project management of industrial facility construction has high challenges and complexity. Various factors influence the effectiveness and risks of multi-project implementation.
Saut B Siahaan, Sofia W. Alisjahbana, Onnyxiforus Gondokusumo   +2 more
doaj   +1 more source

Light Ion Accelerating Line (L3IA): Test Experiment at ILIL-PW

, 2018
The construction of a novel Laser driven Light Ions Acceleration Line(L3IA) is progressing rapidly towards the operation, following the recent upgrade of the ILIL-PW laser facility.
Baffigi, F., Brandi, F., Bussolino, G., Cristoforetti, G., Fazzi, A., Fulgentini, L., Giove, D., Gizzi, L. A., Koester, P., Labate, L., Maero, G., Palla, D., Romé, M., Tomassini, P.   +13 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Development of an Easy-to-Use Tool for the Assessment of Emergency Department Physical Design

Emergency, 2014
Physical design of the emergency department (ED) has an important effect on its role and function. To date, no guidelines have been introduced to set the standards for the construction of EDs in Iran.
Alireza Majidi, Ali Tabatabaey, Hassan Motamed, Maryam Motamedi, Mohammad Mehdi Forouzanfar   +4 more
doaj  

Modernization of the competencies of construction managers in the implementation of projects using BIM [PDF]

BIO Web of Conferences
The use of Building Information Modeling at the construction stage of the facility involves not only the use of new technologies, but also the revision of the functional roles of participants in the implementation of the investment and construction ...
Zharov Iaroslav V.
doaj   +1 more source

Similarity evaluation of the pump simulation loop in STELLA-2 for conservation of mechanical sodium pump characteristics

Nuclear Engineering and Technology, 2023
The STELLA-2 is a large-scale sodium thermal-hydraulic integral effect test facility and supports the development of PGSFR. The facility adopted Pump Simulation Loop System (PSLS) concept for the mechanical sodium pump in the reference reactor to control
Jung Yoon, Jewhan Lee, Jaehyuk Eoh, Hyungmo Kim, Dong Eok Kim   +4 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source