Results 81 to 90 of about 97,962 (296)
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Layout Optimisation of Multi-Frame Process Unit
Chemical Engineering Transactions, 2019 Facility layout is a very important part in the design of a plant, a good layout can greatly reduce the cost of the plant construction and operation, which brings considerable benefits to the investors.
Siyu Xu, Yufei Wang, Xiao Feng
doaj +1 more source
Design and construction of the Fuels and Materials Examination Facility [PDF]
, 1979 Final design is more than 85 percent complete on the Fuels and Materials Examination Facility, the facility for post-irradiation examination of the fuels and materials tests irradiated in the FFTF and for fuel process development, experimental test pin ...
Burgess, C.A.
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FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura +19 more
wiley +1 more source
Analysis Framework for the Interaction Between Lean Construction and Building Information Modelling [PDF]
Building with Building Information Modelling (BIM) changes design and production processes. But can BIM be used to support process changes designed according to lean production and lean construction principles? To begin to answer this question we provide
Owen, Robert +3 more
core +3 more sources
Optimal Capacities in Discrete Facility Location Design Problem
International Journal of Industrial Engineering and Production Research, 2013 Network location models comprise one of the main categories of location models. These models have various applications in regional and urban planning as well as in transportation, distribution, and energy management.
Ali Shahandeh Nookabadi +2 more
doaj
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
, 2006 This Digital Modelling Report incorporates the previous research completed for the FM\ud Exemplar Project utilising the Sydney Opera House as a case study.
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