Results 151 to 160 of about 12,705,637 (166)

Regulation of Factor VIIIa in the Intrinsic Factor Xase

Thrombosis and Haemostasis, 1999
IntroductionHemophilia A, the most common of the severe, inherited bleeding disorders, results from a deficiency or defect in the plasma protein factor VIII. The activated form of the protein serves as an essential cofactor for factor IXa in the conversion of factor X to factor Xa. This surface-bound complex of enzyme and cofactor is referred to as the
P. Fay
semanticscholar   +3 more sources

Characterization of Chemically Crosslinked Human Factor VIIIa

Biochemical and Biophysical Research Communications, 1994
The activity of factor VIIIa was enhanced and stabilized by treatment of factor VIII with the crosslinker disuccinimidyl suberate. The activity was > 200-fold higher compared with that of native factor VIIIa and was stable for at least 15 days at 4 degrees C and pH 7.2.
E, Persson, M, Ezban
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Protein S Down-Regulates Factor VIIIa by Competing with Factor IXa.

Blood, 2006
Abstract Factor VIII functions as a cofactor in the factor Xase complex responsible for phospholipid surface-dependent conversion of factor X to factor Xa by factor IXa. Factor VIIIa, activated form by thrombin and factor Xa, is down regulated by activated protein C (APC), and the reaction is enhanced by the presence of protein S, a ...
Masahiro Takeyama, Keiji Nogami, Kohei Tatsumi, Yuri Fujita, Ichiro Tanaka, Akira Yoshioka, Midori Shima   +6 more
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A group VIIIa ethylene-responsive factor, CmERF4, negatively regulates waterlogging tolerance in chrysanthemum

Journal of Experimental Botany, 2023
Abstract Ethylene-responsive factors (ERF) play an important role in plant responses to waterlogging stress. However, the function and mechanism of action of ERFVIII in response to waterlogging stress remain poorly understood. In this study, we found that expression of the ERF VIIIa gene CmERF4 in chrysanthemum was induced by ...
Chuanwei Li, Likai Wang, Jiangshuo Su, Wenjie Li, Yun Tang, Nan Zhao, La Lou, Xiaoli Ou, Diwen Jia, Jiafu Jiang, Sumei Chen, Fadi Chen   +11 more
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Na+ Site in Blood Coagulation Factor IXa: Effect on Catalysis and Factor VIIIa Binding

Journal of Molecular Biology, 2005
During blood coagulation, factor IXa (FIXa) activates factor X (FX) requiring Ca2+, phospholipid, and factor VIIIa (FVIIIa). The serine protease domain of FIXa contains a Ca2+ site and is predicted to contain a Na+ site. Comparative homology analysis revealed that Na+ in FIXa coordinates to the carbonyl groups of residues 184A, 185, 221A, and 224 ...
Amy E, Schmidt, Jonathan E, Stewart, Akash, Mathur, Sriram, Krishnaswamy, S Paul, Bajaj   +4 more
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Subunit structure of thrombin-activated human factor VIIIa

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988
Proteolytic activation of human Factor VIII (VIIIa) by thrombin was correlated with the generation of a light-chain-derived 73(71) kDa polypeptide plus polypeptides of 51 and 43 kDa derived from the heavy chain(s). Factor VIIIa activity was unstable and decayed to an inactive form (VIIIi) in the absence of additional proteolysis.
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Factor VIII Region 1811–1818 Is Involved in Factor IXa Binding and Factor VIIIa Stability

Blood, 2012
Abstract Abstract 3357 Previously, we identified a role for the lysine residue couple 1967/1968 in the stability of activated factor VIII (FVIIIa). Using tandem mass tags (TMT 126/127) in combination with mass spectrometry, we identified lysine residues involved in the interaction between the A2 domain and the rest of ...
Esther Bloem, Henriet Meems, Maartje van den Biggelaar, Koen Mertens, Alexander B Meijer   +4 more
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Kinetics of Factor X Activation by Factor IXa the Effects of Phospholipid and Factor VIIIa

Thrombosis and Haemostasis, 1979
The kinetics of factor X activation by activated clotting factor IXa was measured either in the presence or absence of phospholipid, Ca-ions and factor VIIIa. This study was carried out with purified bovine clotting factors. The rate of factor Xa formation was measured using the chromogenic substrate S 2222.
G.V. Dieyen, J. Rosing, G. Tans, H.C. Hemker   +3 more
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Factor VIIIa-induced gene expression: potential implications in pathophysiology.

Trends in cardiovascular medicine, 2001
Binding of factor VIIa (VIIa) to tissue factor (TF) triggers the coagulation cascade. Several studies indicate a possible role for TF-VIIa in other cellular functions, in addition to coagulation, under pathophysiological conditions. Recent studies suggest that TF-VIIa mediates cell signaling by two distinct mechanisms, TF cytoplasmic domain-dependent ...
L V, Rao, U R, Pendurthi
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Partial Activation of the Factor VIIIa−Factor IXa Enzyme Complex by Dihexanoic Phosphatidylserine at Submicellar Concentrations

Biochemistry, 1997
Phosphatidylserine (PS)-containing membranes increase the kcat of the factor VIIIa-factor IXa enzyme complex by more than 1000-fold. While PS supports specific, high-affinity membrane binding of factor VIIIa and factor IXa, it is not known whether PS is the lipid that activates the membrane-bound complex.
G E, Gilbert, A A, Arena
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