Results 61 to 70 of about 207,927 (306)

The occurrence and treatment of false positive reactions in enzyme-linked immunosorbent assays (ELISA) for the presence of fungal antigens in clinical samples

open access: yes, 1979
Non-specific positive reactions have been revealed in enzyme-linked immunosorbent assays (ELISA) of sera for the presence of fungal antigen. These false positives were recognized by their occurrence in tests for both Candida albicans and Aspergillus ...
Richardson, Malcolm   +4 more
core   +2 more sources

Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples

open access: yesПроблемы особо опасных инфекций, 2014
CFU/ml) and soluble antigens (FI) − ≥ 4.8 ng/ml and high specificity, confirmed by the absence of false-positive reactions with five heterologous microorganisms. The test-systems were used for Y.
O. A. Noskova   +6 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Fabrication of a Microfluidic Test Device with a 3D Printer and Its Combination with the Loop Mediated Isothermal Amplification Method to Detect Streptococcus pyogenes

open access: yesMicromachines
New rapid, reliable, and cost-effective alternative systems are needed for the rapid diagnosis of Streptococcus pyogenes. The aim of this study was to fabricate a microfluidic test device to detect Streptococcus pyogenes by combining the Loop-mediated ...
Hayriye Kirkoyun Uysal   +2 more
doaj   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

open access: yesFEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik   +6 more
wiley   +1 more source

False‐positive analysis of functional MRI during simulated deep brain stimulation: A phantom study

open access: yes, 2008
Purpose: To investigate the false-positive activations/deactivations in functional MRI (fMRI) of deep brain stimulation (DBS) using a phantom. Materials and Methods: fMRI experiments were performed on a 1.5T scanner using a single-shot gradient-echo echo-
Yuan‐Yu Hsu   +15 more
core   +1 more source

Cognitive and affective reactions to success and failure: The quality of feedback as the determinant of self-verifying and self-enhancing motivation [PDF]

open access: yesPsihologija, 2011
A study was conducted to ascertain what the dominant self motivation is like - selfverifying or self-enhancing? - in a situation when a person is faced with a negative evaluation of a central personality characteristic.
Bodroža Bojana
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Blood Culture Contaminants in a Paediatric Population : Retrospective study from a tertiary hospital in Oman

open access: yesSultan Qaboos University Medical Journal, 2017
Objectives: Most children presenting with febrile illness require a blood culture to determine the causative organism as well as its sensitivity to antibiotics. However, false-positive results lead to unnecessary hospitalisations, prescriptions and tests.
Mohamed A. El-Naggari   +5 more
doaj   +1 more source

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