Results 91 to 100 of about 726,554 (241)

Quantifying data quality in a citizen science monitoring program: False negatives, false positives and occupancy trends

Conservation Science and Practice, 2019
Data collected by volunteers are an important source of information used in species management decisions, yet concerns are often raised over the quality of such data. Two major forms of error exist in occupancy datasets; failing to observe a species when
Sam S. Cruickshank, Christoph Bühler, Benedikt R. Schmidt   +2 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre– and post–RDT introduction

Malaria Journal
Background Pfhrp2 and pfhrp3 deletions are threatening Plasmodium falciparum malaria diagnosis by rapid diagnostic tests (RDT) due to false negatives.
Irene Molina-de la Fuente, M. Andreína Pacheco, Luz García, Vicenta González, Matilde Riloha, Consuelo Oki, Agustín Benito, Ananias A. Escalante, Pedro Berzosa   +8 more
doaj   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Explainable Split-Learning-Based Framework for Accurate Pulmonary Nodule Classification

Bioengineering
Lung cancer rates are the highest among cancers, making it the leading cause of death worldwide. With advances in new technologies and diverse diagnostic methods, Computer-Aided Diagnosis Systems (CADx) have improved pulmonary nodule classification with ...
Amira Bouamrane, Makhlouf Derdour, Ahmed Alksas, Norah Saleh ALghamdi, Mohamed Ghazal, Ayman El-Baz   +5 more
doaj   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Incorporating scale uncertainty in microbiome and gene expression analysis as an extension of normalization

Genome Biology
Statistical normalizations are used in differential analyses to address sample-to-sample variation in sequencing depth. Yet normalizations make strong, implicit assumptions about the scale of biological systems, such as microbial load, leading to false ...
Michelle Pistner Nixon, Gregory B. Gloor, Justin D. Silverman   +2 more
doaj   +1 more source