Results 101 to 110 of about 11,099 (258)

Functional States Recognition System for Fed-batch Cultivation of Saccharomyces cerevisiae [PDF]

Bioautomation, 2008
Free software for entering and documenting data EpiData is here used for design of a system for functional states recognition during a fermentation process.
Pencheva T., Ljakova K.
doaj  

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

Analyzing the Development of a Sustainable Enterprise in an Emerging Economy: Black Cacau and the Bean‐to‐Bar Philosophy

Business Strategy and the Environment, EarlyView.
ABSTRACT This study examines sustainable entrepreneurship (SE) in emerging economies through the case of Black Cacau, a Brazilian bean‐to‐bar enterprise integrating agroforestry, direct trade, and Afro‐Brazilian cultural empowerment. Adapting the Patagonia‐based framework of Allal‐Chérif et al., the study explores how SE operates under conditions of ...
Marcelo Dionisio
wiley   +1 more source

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source

Scalable catalyst production process for oleate hydratase whole‐cell biocatalysis

Biotechnology Progress, EarlyView.
This study establishes a scalable lactose‐induced fed‐batch fermentation for an Escherichia coli whole‐cell biocatalyst containing an oleate hydratase. Operation at a higher growth rate markedly increased biomass yields while maintaining catalyst activity and was demonstrated at 150 L scale.
Rebekka Horstmann, Mario Beckers, Jörn Viell, Fabian Allan Watt, Valentin Steier, Marcel Mann, Jørgen B. Magnus   +6 more
wiley   +1 more source

Screening of bispecific antibodies during single cell cloning of recombinant Chinese hamster ovary cell lines to improve titer and product quality

Biotechnology Progress, EarlyView.
Abstract Development of therapeutic bispecific antibodies (BsAbs) poses significant manufacturability challenges associated with correct chain pairing and an increased diversity of impurities. Here we address this with a product quality assessment during cell line development (CLD), forgoing the need for genetic characterization during cell line ...
Molly Robinson, Jessica Willmott, Holly Corrigall, Daniela Lega, Joseph Stirling, Vithiya Vimalraj, Mitul Patel, Robyn Emmins   +7 more
wiley   +1 more source

Regenerative perfusion bioreactor for biologics production with lower COGs

Biotechnology Progress, EarlyView.
Abstract Perfusion bioreactor systems enable intensified bioprocessing with high cell densities and volumetric productivities in monoclonal antibody (mAb) manufacturing. However, their widespread adoption remains constrained by the high consumption of chemically defined cell culture media.
Eric Wynne, Dohyun Park, Hourieh Ahi, Jaeweon Lee, Seongkyu Yoon, Jongyoon Han   +5 more
wiley   +1 more source

Parallelized microscale fed-batch cultivation in online-monitored microtiter plates: implications of media composition and feed strategies for process design and performance. [PDF]

J Ind Microbiol Biotechnol, 2020
Morschett H, Jansen R, Neuendorf C, Moch M, Wiechert W, Oldiges M.   +5 more
europepmc   +1 more source

Determining the Nutritional Potential of Ancient Wheat Species: A Closer Look at Starch and Protein Digestibility, and Phenolic Acid Content

Cereal Chemistry, EarlyView.
ABSTRACT Background and Objectives The recent trend towards healthy food choices and sustainable agriculture has revived interest in underutilized wheat species such as emmer, einkorn, and spelt. Given these developments, the objective of the study was to compare the differences in chemical composition and digestibility characteristics among ...
Sathsara Deyalage, Sijo Joseph Thandapilly, James Duncan House, Adam Franczyk, Amanda Gomes Almeida Sá, Maneka Malalgoda   +5 more
wiley   +1 more source

Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy. [PDF]

Front Microbiol, 2020
Zhang C, Ma Y, Miao H, Tang X, Xu B, Wu Q, Mu Y, Huang Z.   +7 more
europepmc   +1 more source