Results 91 to 100 of about 11,099 (258)

Glycoengineered Recombinant Alpha1‐Antitrypsin Results in Comparable In Vitro and In Vivo Activities to Human Plasma‐Derived Protein

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Alpha‐1‐antitrypsin (A1AT) is a multifunctional, clinically important, high‐value therapeutic glycoprotein that can be used for the treatment of many diseases, such as A1AT deficiency, diabetes, graft‐versus‐host disease, cystic fibrosis, and various viral infections. Currently, the only U.S. food and drug administration‐approved treatment for
Frances Rocamora, Chen‐Lin Hsieh, Sanne Schoffelen, Johnny Arnsdorf, Eric A. Toth, Abdul S. Yunus, Thomas E. Cleveland IV, Sara Petersen Bjørn, Mina Ying Min Wu, Noel G. McElvaney, Bjørn Gunnar Rude Voldborg, Thomas R. Fuerst, Nathan E. Lewis   +12 more
wiley   +1 more source

Glucose-Limited Fed-Batch Cultivation Strategy to Mimic Large-Scale Effects in Escherichia coli Linked to Accumulation of Non-Canonical Branched-Chain Amino Acids by Combination of Pyruvate Pulses and Dissolved Oxygen Limitation. [PDF]

Microorganisms, 2021
García ÁC, Hauptmann P, Neubauer P.
europepmc   +1 more source

Translating Blue Light Stimulation From Batch to Perfusion: Process and Intracellular Metabolic Analysis

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Improving cell‐specific productivity remains a central objective in biopharmaceutical manufacturing. In this study, the effect of blue light illumination on IgG1‐producing CHO DP‐12 cells was systematically evaluated across batch, fed‐batch, and perfusion cultivations in controlled 3 L bioreactor systems.
Stefanie Föller, Attila Teleki, Ralf Takors   +2 more
wiley   +1 more source

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The high cost of cultivation media remains one of the barriers to the commercialization of cultivated meat. Current serum‐free media reduce reliance on fetal bovine serum but only achieve modest cost savings. Especially at larger scales (> 20 m3), pharmaceutical‐grade amino acids and recombinant proteins remain major cost drivers.
Jelle C. Hoek, Jean‐Marc Daran, Marieke E. Klijn, Marcel A. Vieira‐Lara   +3 more
wiley   +1 more source

Brunovsky Normal Form of Monod Kinetics Models and Growth Rate Control of a Fed-batch Cultivation Process [PDF]

Bioautomation, 2007
A mathematical methodology that gives assistance to design of fed-batch stabilization and control is presented. The methodology is based both on Utility theory and optimal Control theory. The Utility theory deals with the expressed subjective preferences
Pavlov Y.
doaj  

LLM‐Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Mechanistic models of Chinese Hamster Ovary (CHO) cell cultures are essential for efficient bioprocess development and control, yet parameter estimation remains a critical challenge in model calibration. As model complexity increases, the number of parameters grows and their values become highly system‐specific due to biological variability ...
Han Bit Kim, Janghan Lee, Seo‐Yeon Kim, Jihoon Kim, Moo Sun Hong   +4 more
wiley   +1 more source

Pectinase‐Based Bioprocesses for Circular Bioeconomy and Sustainable Industrial Development

Biotechnology and Bioengineering, EarlyView.
This picture demonstrates a unified strategy for sustainable pectinase production, focusing on microbial bioproduction, innovative strategies in enzyme engineering (Protein engineering, rational enzyme design, directed evolution and heterologous expression) and various applications in industry, including food, textiles, pulp and paper, agriculture and ...
Nisha Sharma, Dimple Tanwar, Usha Grewal, Murugesan Mohana Keerthi, Yogesh K. Ahlawat, Nitin Pandey, Chirag G. Makvana   +6 more
wiley   +1 more source

High Cell Density Cultivation of HEK293 Cells Using a 2L Membrane‐Stirred Bioreactor

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The use of perfusion systems for the continuous cultivation of animal cells at high cell density (HCD) for the production of recombinant proteins, viruses, and viral vectors has many advantages. Besides an enhancement of volumetric productivity and high cell viability, the product quality can be improved through a steady‐state environment ...
Lennart Jacobtorweihe, Yasemin van Heuvel, Kathrina Scheiermann, Patrick Bongartz, Yvonne Genzel, Udo Reichl   +5 more
wiley   +1 more source

Expanding Metabolic Online Monitoring Capabilities in 96‐Deepwell Microtiter Plates by Combining Optoelectronic O2 and CO2 Sensors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT To increase the information content in early stages of bioprocess development, various technologies have been introduced for monitoring cultivations in microtiter plates (MTPs). Besides spectroscopical measurements, like in the BioLector technology, optical measurements with luminescent sensors, placed directly in the cultivation medium ...
Robert Dinger, David Pfeifer, David Flitsch, Jan P. Fischer, Clemens Lattermann, Sergey M. Borisov, Jochen Büchs   +6 more
wiley   +1 more source

Tailoring the properties of rhamnose-rich exopolysaccharides from cheese whey via continuous fed-batch fermentation for active packaging applications

Carbohydrate Polymer Technologies and Applications
This study explores the sustainable bioconversion of cheese whey (CW) into high-value exopolysaccharides (EPS) by Halomonas caseinilytica K1 through the optimization of bioreactor operation modes.
Samia Azabou, Ichrak Joulak, Patrícia Concórdio-Reis, Cristiana AV Torres, Chantal Sevrin, Christian Grandfils, Hamadi Attia, Adem Gharsallaoui, Filomena Freitas   +8 more
doaj   +1 more source