Results 261 to 270 of about 19,074 (293)

Fed-Batch cultivation ofSaccharomyces cerevisiae with increased content of Δ5,7-sterols

Folia Microbiologica, 1986
Cultivation of Saccharomyces cerevisiae with a linear nutrient feed was used to test the validity of a mathematical model describing changes in the physiological state of the culture. Markers of these changes were the concentrations of proteins and delta 5,7-sterols in yeast dry mass.
B, Bĕhalová, J, Votruba, A, Pichová, K, Beran   +3 more
openaire   +2 more sources

EXPERIMENTAL INVESTIGATION OF DATADRIVEN MODELLING FOR CONTROL OF FED-BATCH CULTIVATION

IFAC Proceedings Volumes, 2005
Abstract Application of the data driven modelling framework entitled Grid of Linear Models to derive a predictive model for a fed-batch fermentation process is presented. The modelling framework utilises a large number of local linear models to approximate the behaviour of the non-linear process. Industrial data from a fed-batch fermentation has been
Jan Kamyno Rasmussen, Sten Bay Jørgensen   +1 more
openaire   +1 more source

A Guide to Fed-Batch Cultivation of Pichia pastoris on Laboratory Scale

Fed-batch is the most commonly used cultivation mode for industrial production of recombinant proteins with Pichia pastoris. On a laboratory scale, fed-batch culture provides a way to control the specific biomass growth rates at any pre-set value, allowing the conditions of biomass growth and recombinant product formation to be systematically studied ...
Marcel, Straumann, Hana, Raschmanová, Valentina, Haag, Iwo, Zamora, Karin, Kovar   +4 more
openaire   +2 more sources

The Hagen–Poiseuille pump for parallel fed-batch cultivations in microbioreactors

Chemical Engineering Science, 2009
Abstract We developed and applied a micropump for liquid flows of 0–150 nL/min. The operating principle of this pump is based on the Hagen–Poiseuille law. The main advantages of this pump are that an accurate continuous flow rate can be achieved, it is cheap to manufacture, easy to parallelize and easy to integrate in microtiter plates.
Michiel van Leeuwen, Nicolaas A.A. Buijs, André B. Canelas, Arthur Oudshoorn, Joseph J. Heijnen, Walter M. van Gulik   +5 more
openaire   +1 more source

Genetic Algorithms Based Parameter Identification of Yeast Fed-Batch Cultivation

2011
Different kinds of genetic algorithms have been investigated for a parameter identification of a fermentation process. Altogether eight realizations of genetic algorithms have been presented - four of simple genetic algorithms and four of multi-population ones.
Maria Angelova, Stoyan Tzonkov, Tania Pencheva   +2 more
openaire   +1 more source

Recombinant streptokinase production by fed-batch cultivation of Escherichia coli

Enzyme and Microbial Technology, 1999
Abstract Streptokinase (SK) is a specific effective medicine for thrombolytic therapy of acute myocardial infarction. This study established a process for the pilot production of recombinant streptokinase (r-SK). Engineering bacteria were fermented in a 20-l fermentor to produce r-SK. After simple renaturation and purification, 12.9 g of r-SK with 97.
Xue-Wu Zhang, Tao Sun, Xiao-Ni Huang, Xin Liu, De-Xiang Gu, Zhan-Qui Tang   +5 more
openaire   +1 more source

Designing new controller for fed-batch cultivation of recombinant E.coli

2008 27th Chinese Control Conference, 2008
Fed-batch fermentation processes are common methods of producing biologic recombinant cells from different microorganisms. Model-based control of bioprocesses is a difficult task due to the challenges associated with bioprocess modelling and the lack of on-line measurements.
Mohseni Saleh, Vali Ahmad Reza, Babaeipour Valiollah   +2 more
openaire   +1 more source

Yield Improvements Utilising Fed-Batch Cultivation and Optimisation Strategies

1998
In previous work, we have demonstrated the improvements that fed-batch systems can bring to the overall antibody productivity and antibody concentration obtained in batch culture.
C. S. Sanderson, C. P. Marquis, J. P. Barford   +2 more
openaire   +1 more source

Control of Fed-Batch Yeast Cultivation Using a Capacitance Sensor

2009
Abstract Saccharomyces cerevisiae biomass is still one of the most important biotechnological products in the world, mainly in the form of baker's yeast. Costs of substrates have an important position in the overall economics of baker's yeast production, which is usually run in fed-batch bioreactors.
Giann B. Reis, Antonio C.L. Horta, Teresa C. Zangirolami, Roberto C. Giordano, Antonio J.G. Cruz   +4 more
openaire   +1 more source

Grey-box stochastic modelling of industrial fed-batch cultivation

2006
Abstract This paper presents application of a grey-box stochastic modelling framework for developing continuous time models for dynamic systems based on experimental data. The framework is used to develop predictive models suited for control purposes.
Jan K. Rasmussen, Henrik Madsen, Sten B. Jørgensen   +2 more
openaire   +1 more source