Results 251 to 260 of about 19,074 (293)
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Probing control of fed-batch cultivations: analysis and tuning
Control Engineering Practice, 2001Production of various proteins can today be made using genetically modified Escherichia coli bacteria. In cultivations of E. coli it is important to avoid accumulation of the by-product acetate. Formation of acetate occurs when the specific glucose uptake exceeds a critical value and can be avoided by a proper feeding strategy. A difficulty is that the
M. Åkesson, P. Hagander, J.P. Axelsson
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A fed‐batch cultivation method for the simultaneous utilization of glucose and formate by yeasts
Acta Biotechnologica, 1988AbstractA fed‐batch cultivation method was elaborated which 1. warrants simultaneous consumption of glucose and formate, 2. is easy to employ and provides reproducible results, 3. gives information about the role of formate in yeast growth, 4. results in almost identical conditions for parallel cultures on glucose and glucose/formate.
R. H. Müller, E. Zimmermann, W. Babel
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Optimal fed‐batch cultivation when mass transfer becomes limiting
Biotechnology and Bioengineering, 2007AbstractIn the design of an aerobic fed‐batch process to produce, for example, a pharmaceutical protein, the volumetric production rate will eventually become limited by mass transfer when the biomass concentration exceeds a certain upper limit x*. It appears to be common practice to switch from exponential feed of substrate to a constant feed rate ...
John, Villadsen, Kiran R, Patil
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2013
Fed-batch reactor is based on feeding of a growth limiting nutrient substrate to a culture. Cell growth and fermentation can be controlled by the feeding strategy. The fed-batch strategy is typically used to reach a high cell density in the bioreactor or mimic a continuous culture. Mostly the feed solution is highly concentrated.
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Fed-batch reactor is based on feeding of a growth limiting nutrient substrate to a culture. Cell growth and fermentation can be controlled by the feeding strategy. The fed-batch strategy is typically used to reach a high cell density in the bioreactor or mimic a continuous culture. Mostly the feed solution is highly concentrated.
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Clavulanic Acid Degradation in Streptomyces clavuligerus Fed‐Batch Cultivations
Biotechnology Progress, 2002Abstract Clavulanic acid (CA) is an important antibiotic that is produced by Streptomyces clavuligerus . CA is unstable and product degradation has turned out to have a major impact on product titers in fed‐batch cultivations.
Johannes A, Roubos +4 more
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Change in hyphal morphology of Aspergillus oryzae during fed-batch cultivation
Applied Microbiology and Biotechnology, 2006Industrial enzymes are often produced by filamentous fungi in fed-batch cultivations. During cultivation, the different morphological forms displayed by the fungi have an impact on the overall production. The morphology of a recombinant lipase producing Aspergillus oryzae strain was investigated during fed-batch cultivations.
Martin B, Haack +3 more
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Cultivation of Arthrospira (Spirulina) platensis by Fed-Batch Process
2012This chapter comments on fed-batch cultivation of Arthrospira platensis under different carbon and nitrogen sources, pH, temperature, light intensity, type of photobioreactor and typical parameters of the fed-batch process, such as feeding time, addition protocol and flow rate.
João C. M. Carvalho +3 more
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Construction and fed-batch cultivation of Candida famata with enhanced riboflavin production
Journal of Biotechnology, 2014Riboflavin (vitamin B2) is an essential nutrition component serving as a precursor of coenzymes FMN and FAD that are involved mostly in reactions of oxidative metabolism. Riboflavin is produced in commercial scale and is used in feed and food industries, and in medicine.
Kostyantyn, Dmytruk +6 more
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Assessment of fed-batch cultivation strategies for an inducible CHO cell line
Journal of Biotechnology, 2019In order to maximize cell growth and productivity for an inducible CHO cell line expressing rituximab, various fed-batch culture strategies were investigated. In each case, the performance was evaluated for cultures induced at moderate and high cell density conditions (4 × 106 and 10 × 106 cells/mL) to assess the impact of the timing of induction.
Kahina Mellahi +6 more
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Evaluation of mammalian fed-batch cultivations by two different models
Bioprocess Engineering, 1997Simulations of fed-batch hybridoma cell cultures were performed with two different models, a new model suggested by Nielsen and an older model suggested by Batt and Kompala. The response of the new Nielsen model was less sensitive to changes in feed concentrations and frequencies than the Batt and Kompala model.
A. Ryszczuk, C. Emborg
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