Results 41 to 50 of about 2,478,172 (300)

Mutation Breeding and Optimization of Fermentation Conditions of Bacillus Highly Producing Antimicrobial Lipopeptide Fengycin

Shipin gongye ke-ji, 2023
In order to increase the yield of Fengycin, Bacillus YA-215 was used as the starting strain in this study to obtain high-yield Fengycin mutants through compound mutagenesis (UV mutagenesis, ARTP-LiCl mutagenesis) breeding.
Shangli CHEN, Futian YU, Yuanyuan SHEN, Xiaoling LIU   +3 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Efficient Production of γ-CD from Starch by γ-CGTase Heterologously Produced in Pichia pastoris, Assisted by β-CGTase Liquefaction and Pullulanase Debranching

Molecules
Cyclodextrins (CDs) are cyclic oligosaccharides composed of α(1 → 4) linked glucose units, which are widely used as solubilizers and stabilizers in the food, pharmaceutical and cosmetic industries.
Nuo Chen, Xiaoxiao Li, Zhengyu Jin, Birte Svensson, Yuxiang Bai   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Integrated RSM and Genomic Analysis for Optimized Sporulation in Heyndrickxia coagulans

Fermentation
Industrial spore production of the probiotic Heyndrickxia coagulans is hindered by its generally low and highly variable sporulation efficiency across strains.
Yiwei Jin, Feng Chen, Jiang Cao
doaj   +1 more source

Optimization of Potato Starch Residue L-Lactic Fermentation Culture Conditions

, 2011
Potato starch residue was used as raw material , a single factor test was used to determine the lactobacillus casei L-lactic fermentation in the amounts of CaCO3 addition, fermentation temperature, residue saccharification of starch concentration, the ...
Dan Zhao, Zhong Xu, Nan Liu, Duo Wang
core   +1 more source

Diversity of yeasts involved in the fermentation of tchoukoutou, an opaque sorghum beer from Benin [PDF]

, 2011
Opaque sorghum beers are traditional alcoholic beverages in several African countries. Known as tchoukoutou in Benin, the beer is often obtained from an uncontrolled fermentation. It is consumed in an actively fermenting state and has a sour taste.
Hounhouigan, A.J.D., Nout, M.J.R., Boekel, M.A.J.S., van, Vieira-Dalode, G., Kayode, A.P.P., Linnemann, A.R., Kotchoni, S.O.   +6 more
core   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Impact of molasses quality on ethyl alcohol fermentation by the yeast saccharomyces cerevisiae [PDF]

, 2009
The use of blackstrap cane molasses in industrial ethanol production is characterised by fluctuations in fermentation efficiency due to its compositional variation.
Sibanda, Mkhululi Benedict
core   +1 more source

Bacillus velezensis B313-6 as a Potential Biocontrol Strain Against Anthracnose of Panax quinquefolius, and Its Fermentation Optimization, and Field Efficacy on American Ginseng

Agriculture
Anthracnose of American ginseng is caused by Colletotrichum panacicola. In this study, Bacillus velezensis B313-6, isolated from the rhizosphere soil of American ginseng, was identified based on morphological, physiological, biochemical, 16S rRNA, and ...
Shuang Feng, Yue Shi, Ruijie Liu, Xin Li, Shuai Shao, Nan Yin, Yuejia Song, Haoxin Zhan, Yuxin He, Tom Hsiang, Changqing Chen, Liping Liu, Jie Gao   +12 more
doaj   +1 more source