Evolutionary dynamics determines adaptation to inactivation of an essential gene [PDF]
Genetic inactivation of essential genes creates an evolutionary scenario distinct from escape from drug inhibition, but the mechanisms of microbe adaptations in such cases remain unknown. Here we inactivate E. coli dihydrofolate reductase (DHFR) by introducing D27G,N,F chromosomal mutations in a key catalytic residue with subsequent adaptation by ...
arxiv
Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme [PDF]
Iron-peroxide intermediates are central in the reaction cycle of many iron-containing biomolecules. We trapped iron(III)-(hydro)peroxo species in crystals of superoxide reductase (SOR), a nonheme mononuclear iron enzyme that scavenges superoxide radicals.
arxiv
Involvement of the Flavin si-Face Tyrosine on the Structure and Function of Ferredoxin-NADP+ Reductases [PDF]
Adrián K. Arakaki+4 more
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Probing the Determinants of Coenzyme Specificity in Ferredoxin-NADP+ Reductase by Site-directed Mutagenesis [PDF]
Milagros Medina+7 more
openalex +1 more source
Ferredoxin-NADP+ Reductase and Ferredoxin of the Protozoan Parasite Toxoplasma gondii Interact Productivelyin Vitro and in Vivo [PDF]
V. Pandini+5 more
openalex +1 more source
Anabaena sp. PCC 7119 Flavodoxin as Electron Carrier from Photosystem I to Ferredoxin-NADP+Reductase [PDF]
José L. Casaus+7 more
openalex +1 more source
Involvement of the Pyrophosphate and the 2′-Phosphate Binding Regions of Ferredoxin-NADP+ Reductase in Coenzyme Specificity [PDF]
Jesús Tejero+9 more
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The marine unicellular green cyanobacterium Prochlorococcus marinus MIT9313 belongs to the most abundant and photosynthetically productive genus of cyanobacteria in the oceans.
Jesús Barrera-Rojas+4 more
doaj
The oxidant‐responsive diaphorase of Rhodobacter capsulatus is a ferredoxin (flavodoxin)‐NADP(H) reductase [PDF]
Cristian Bittel+4 more
openalex +1 more source
The photo‐enzyme coupling system (PECS) holds immense potential in “green” biomanufacturing, encompassing the realms of pharmaceuticals, fuels, and carbon sequestration.
Yan Li+6 more
doaj +1 more source