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Field-Flow Fractionation in Molecular Biology and Biotechnology [PDF]

open access: yesMolecules, 2023
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension.
Stefano Giordani   +5 more
doaj   +2 more sources

Understanding the Degradation of Core-Shell Nanogels Using Asymmetrical Flow Field Flow Fractionation [PDF]

open access: yesJournal of Functional Biomaterials, 2023
Nanogels are candidates for biomedical applications, and core-shell nanogels offer the potential to tune thermoresponsive behaviour with the capacity for extensive degradation.
Edyta Niezabitowska   +5 more
doaj   +2 more sources

Proteomics protocol for obtaining extracellular vesicle from human plasma using asymmetrical flow field-flow fractionation technology [PDF]

open access: yesSTAR Protocols, 2023
Summary: Plasma extracellular vesicles (EVs) represent a potential resource for biomarkers of multiple diseases. Here, we present a protocol for obtaining EVs from human plasma using asymmetrical flow field-flow fractionation technology.
Na Li   +4 more
doaj   +2 more sources

The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery [PDF]

open access: yesMolecules, 2023
Asymmetric-flow field-flow fractionation (AF4) is a gentle, flexible, and powerful separation technique that is widely utilized for fractionating nanometer-sized analytes, which extend to many emerging nanocarriers for drug delivery, including lipid ...
Juan Bian   +3 more
doaj   +2 more sources

Native RNA Purification Method for Small RNA Molecules Based on Asymmetrical Flow Field-Flow Fractionation [PDF]

open access: yesPharmaceuticals, 2022
RNA molecules provide promising new possibilities for the prevention and treatment of viral infections and diseases. The rapid development of RNA biology and medicine requires advanced methods for the purification of RNA molecules, which allow fast and ...
Alesia A. Levanova   +5 more
doaj   +2 more sources

Flow field-flow fractionation coupled with multidetector: A robust approach for the separation and characterization of resistant starch [PDF]

open access: yesFood Chemistry: X
The unique properties of resistant starch (RS) have made it applicable in the formulation of a broad range of functional foods. The physicochemical properties of RS play a crucial role in its applications.
Mu Wang   +5 more
doaj   +2 more sources

Assessment of the Effects of Structural Modification of Gastrodia elata Polysaccharide on Anti-Breast Cancer Activity Using Asymmetrical Flow Field-Flow Fractionation [PDF]

open access: yesMolecules, 2023
Gastrodia elata (“Tian Ma” in Chinese) is used as a food and medical ingredient in traditional Chinese medicine. In this study, to enhance the anti-breast cancer activity of Gastrodia elata polysaccharide (GEP), GEPs were modified via sulfidation (SGEP ...
Xiaoying Liu   +7 more
doaj   +2 more sources

Dealing with the complexity of conjugated and self‐assembled polymer‐nanostructures using field‐flow fractionation [PDF]

open access: yesAnalytical Science Advances, 2021
Broad diversity and heterogeneity are inherently showcased by both natural and synthetic macromolecular structures. The high application potential for such structures and their combinations calls for novel analytical approaches that allow for ...
Upenyu L. Muza   +2 more
doaj   +2 more sources

Cascade Filtration With PCR Detection and Field-Flow-Fractionation Online With ICP-MS for the Characterization of DNA Interaction With Suspended Particulate Matter [PDF]

open access: yesFrontiers in Chemistry, 2022
The variety of applied antibiotics in animal and human medicine results in the release, development, and spread of relevant numbers of antibiotic resistance genes (ARGs) in the environment.
Volker Nischwitz   +3 more
doaj   +2 more sources

Revisiting the dynamics of proteins during milk powder hydration using asymmetric flow field-flow fractionation (AF4) [PDF]

open access: yesCurrent Research in Food Science, 2021
The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang   +4 more
doaj   +2 more sources

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