Surface Characterization of Biocompatible Channels for Field-Flow Fractionation of Biological Samples [PDF]
Biocompatible methods capable of rapid purification and fractionation of analytes from complex natural matrices are increasingly in demand, particularly at the forefront of biotechnological applications.
RESCHIGLIAN, PIERLUIGI +9 more
core
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Fast optimal control of asymmetric flow field flow fractionation processes [PDF]
S.207-213We present optimization problem for Asymmetric Flow Field Flow Fractionation, which is a widely used technique for segregation of two or more particles of submicron scale, according to their hydrodynamic radius.
Pinnau, R., Bayramov, N., Nagapetyan, T.
core +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Deterministic ratchets for suspension fractionation [PDF]
Driven by the current insights in sustainability and technological development in biorefining natural renewable resources, the food industry has taken an interest in fractionation of agrofood materials, like milk and cereal crops.
Kulrattanarak, T.
core
Efficient algorithms for asymmetric flow field flow fractionation [PDF]
This thesis is devoted to the modeling and simulation of Asymmetric Flow Field Flow Fractionation, which is a technique for separating particles of submicron scale.
Nagapetyan, T.
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Design and analysis strategies for robust microbiome ageing research
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Asymmetrical flow field-flow-fractionation in pharmaceutical analytics [PDF]
Due to enormous progress in recombinant DNA techniques and methodology, a multitude of biosynthetic, pharmaceutically relevant polypeptides and proteins became available in the past decade and have been employed in numerous pharmaceutical products ...
Fraunhofer, Wolfgang
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Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Hollow-fiber flow field-flow fractionation: A pipeline to scale down separation and enhance detection of proteins and cells [PDF]
Commercial flow field-flow fractionation (FlFFF) employs macro-scale, flat-type channels. The idea of hollow-fiber (HF) membranes as tubular, micro-column channels for FlFFF (HF FlFFF or, more shortly, HF5) dates back to 1974, with fundamentals on HF5 ...
Rambaldi D. C. +4 more
core +1 more source

