Results 51 to 60 of about 887,918 (338)

Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2019
In asymmetrical flow field-flow fractionation (AF4), similar to other separation techniques, mass recovery and overloading require special attention in order to obtain quantitative results.
M. Marioli, W. Kok
semanticscholar   +1 more source

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

Separations, 2018
Casein is the major protein fraction in milk, and its cross-linking has been a topic of scientific interest for many years. Enzymatic cross-linking has huge potential to modify relevant techno-functional properties of casein, whereas non-enzymatic cross ...
Norbert Raak, Raffaele Andrea Abbate, Albena Lederer, Harald Rohm, Doris Jaros   +4 more
doaj   +1 more source

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

Separation and Detection of Escherichia coli and Saccharomyces cerevisiae Using a Microfluidic Device Integrated with an Optical Fibre

Biosensors, 2019
This paper describes the development of an integrated system using a dry film resistant (DFR) microfluidic channel consisting of pulsed field dielectrophoretic field-flow-fractionation (DEP-FFF) separation and optical detection.
Mohd Firdaus Kamuri, Zurina Zainal Abidin, Mohd Hanif Yaacob, Mohd Nizar Hamidon, Nurul Amziah Md Yunus, Suryani Kamarudin   +5 more
doaj   +1 more source

Development of an advanced on-line position-specific stable carbon isotope system and application to methyl tert-butyl ether [PDF]

, 2009
We present an advanced system for on-line position-specific carbon isotope analysis. The main limitation of on-line intramolecular isotope ratio measurements has been that optimal pyrolytic fragments are obtained mostly at temperatures where the analyte ...
Abelson, Barrie, Barth, Benson, Coplen, Corso, Corso, Corso, Daly, Dempster, Dias, Elsner, Elsner, Fischer, Gray, Hall, Hall, Harrington, Hayes, Hirschorn, Huang, Hunkeler, Jasper, Jeffrey, Kolhatkar, Kuder, Mancini, Mariotti, Matthews, Meckenstock, Meier-Augenstein, Meier-Augenstein, Merritt, Miller, Monson, Morrison, O'Sullivan, Poulson, Richnow, Rosell, Sacks, Sano, Schmidt, Schmidt, Schüth, Slater, Slater, Slater, Smallwood, Somsamak, Somsamak, Vogler, Wang, Wolyniak, Wolyniak, Yamada, Zwank   +56 more
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

STAR Protocols, 2021
Summary: We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected ...
Linda Bojmar, Han Sang Kim, Gabriel C. Tobias, Fanny A. Pelissier Vatter, Serena Lucotti, Kofi Ennu Gyan, Candia M. Kenific, Zurong Wan, Kyung-A Kim, DooA Kim, Jonathan Hernandez, Virginia Pascual, Todd E. Heaton, Michael P. La Quaglia, David Kelsen, Tanya M. Trippett, David R. Jones, William R. Jarnagin, Irina R. Matei, Haiying Zhang, Ayuko Hoshino, David Lyden   +21 more
doaj   +1 more source

Holographic optical trapping

, 2005
Holographic optical tweezers use computer-generated holograms to create arbitrary three-dimensional configurations of single-beam optical traps useful for capturing, moving and transforming mesoscopic objects.
Allen, Alonzo, Arlt, Ashkin, Ashkin, Crocker, Curtis, Curtis, Dufresne, Dufresne, Gahagan, Garces-Chavez, Grier, Guo, He, He, Hughes, Jesacher, Jordan, Korda, Koss, Ladavac, Ladavac, Leach, Liesener, MacDonald, Meister, Melville, Mogensen, Neto, O'Neil, Polin, Reicherter, Reimann, Roichman, Sacks, Sasaki, Simpson, Sinclair, Soskin, Sundbeck   +40 more
core   +1 more source

Isotope (¹⁴C and ¹³C) analysis of deep peat CO₂ using a passive sampling technique [PDF]

, 2009
We developed and tested a new method to collect CO₂ from the surface to deep layers of a peatland for radiocarbon analysis. The method comprises two components: i) a probe equipped with a hydrophobic filter that allows entry of ...
Garnett, M.H., Hardie, S.M.L.
core   +1 more source