Results 81 to 90 of about 60,638 (294)

Genome-Wide Screening and Stability Verification of the Robust Internal Control Genes for RT-qPCR in Filamentous Fungi

, 2022
In real-time quantitative PCR (RT-qPCR), internal control genes (ICGs) are crucial for normalization. This study screened 6 novel ICGs: Pre-mRNA-splicing factor cwc15 (Cwf15); ER associated DnaJ chaperone (DnaJ); E3 ubiquitin-protein ligase NEDD4 (HUL4);
Zhuohan Jing, Jun Ye, Mengyu Chen, Yongxin Tao, Yayong Yang, Xiaoyu Li, Chen, Mengyu, Jing, Zhuohan, Wang, Tengyun, Yang, Yayong, Li, Hui, Xinyu Xu, Lei Shi, Xu, Xinyu, Xie, Baogui, Li, Xiaoyu, Shi, Lei, Hui Li, Ye, Jun, Tao, Yongxin, Tengyun Wang, Baogui Xie   +21 more
core   +1 more source

Enhancing the nutritional profile of de‐oiled rice bran through solid‐state fermentation with Aspergillus oryzae

Food Biomacromolecules, EarlyView.
Abstract An experiment was conducted to explore the impact of fermenting de‐oiled rice bran (DORB) with Aspergillus oryzae on its nutrient composition and anti‐nutritional factors. DORB was inoculated with 7‐day‐old A. oryzae culture grown on potato dextrose agar. The experiment was conducted in triplicate for 120 h, with samples collected at 0, 24, 48,
Amit Ranjan, Narottam Prasad Sahu, Sarvendra Kumar, Ashutosh Dharmendra Deo   +3 more
wiley   +1 more source

Fungal Tolerance: An Alternative for the Selection of Fungi with Potential for the Biological Recovery of Precious Metals

Applied Sciences, 2020
The behavior of various filamentous fungi in the presence of metals such as Cu, Zn, Ni, Fe, Mn, and V has been widely reported. However, there is little information regarding metals such as Au, Ag and Pt that are not in the form of nanoparticles.
Rosalba Argumedo-Delira, Mario J. Gómez-Martínez, Ramiro Uribe-Kaffure   +2 more
doaj   +1 more source

Standardized Protocol for Isolation and Cryopreservation of Cultivable Endophytes From Fresh and Dried Citrus aurantium Peels

Food Safety and Health, EarlyView.
A protocol for converting Citrus aurantium peel—from both fresh fruit and dried Zhike—into a curated endophytic microbial library through standardized surface sterilization, tissue homogenization, and multi‐medium culturing, followed by 16S/ITS‐based identification and −80°C cryopreservation, enabling reproducible exploration of citrus peel microbiota ...
Hujing Cao, Xinlin Huang, Han Yang, Manxi Wu, Jingping Cao, Chongde Sun, Yue Wang   +6 more
wiley   +1 more source

A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi.

PLoS ONE, 2015
The number of fully sequenced fungal genomes is rapidly increasing. Since genetic tools are poorly developed for most filamentous fungi, it is currently difficult to employ genetic engineering for understanding the biology of these fungi and to fully ...
Christina S Nødvig, Jakob B Nielsen, Martin E Kogle, Uffe H Mortensen   +3 more
doaj   +1 more source

Binuclear Copper‐Dependent Oxidative Enzymes Involved in Fungal Natural Product Modifications

Journal of the Chinese Chemical Society, EarlyView.
This article summarizes recent biochemical characterizations of a new enzyme family named by the authors as binuclear copper‐dependent oxidative enzymes (BiNCOs). Found in fungal natural product biosynthesis, BiNCOs catalyze diverse CH functionalization reactions, including C(sp3)H halogenation, C(sp3)H hydroxylation, C(sp3)O macrocyclization, and ...
Chen‐Yu Chiang, Masao Ohashi, Yi Tang
wiley   +1 more source

Periodontitis and atherosclerotic cardiovascular disease: A critical appraisal

Periodontology 2000, EarlyView., 2023
Abstract In spite of intensive research efforts driving spectacular advances in terms of prevention and treatments, cardiovascular diseases (CVDs) remain a leading health burden, accounting for 32% of all deaths (World Health Organization. “Cardiovascular Diseases (CVDs).” WHO, February 1, 2017, https://www.who.int/news‐room/fact‐sheets/detail ...
Maria Clotilde Carra, Hélène Rangé, Giuseppina Caligiuri, Philippe Bouchard   +3 more
wiley   +1 more source

Toward Sustainable Enzyme Production From Agro‐Industrial Residues: Optimizing pH and Soyhull Particle Size in Fungal Fermentation

Journal of Surfactants and Detergents, EarlyView.
Sustainable enzyme production from soyhull fermentation. ABSTRACT Carbohydrase enzymes are critical to sustainable detergent formulations, enabling effective cleaning at lower temperatures and reduced resource use. To improve the economic and environmental viability of enzyme production, this study explored soybean hulls—a low‐value agricultural by ...
Ashwin Sancheti, Lu‐Kwang Ju
wiley   +1 more source

Development of a versatile and conventional technique for gene disruption in filamentous fungi based on CRISPR-Cas9 technology

Scientific Reports, 2017
Filamentous fungi represent an invaluable source of pharmaceutically active compounds. The development of versatile methods to genetically manipulate filamentous fungi is of great value for improving the low yields of bioactive metabolites and expanding ...
Yan-Mei Zheng, Fu-Long Lin, Hao Gao, Gen Zou, Jiang-Wei Zhang, Gao-Qian Wang, Guo-Dong Chen, Zhi-Hua Zhou, Xin-Sheng Yao, Dan Hu   +9 more
doaj   +1 more source

Fungal biotechnology

, 2023
Filamentous fungi are an amazing group of microorganisms able to both degrade and produce a plethora of different compounds. Many fungi have rather modest nutritional requirements, making them very interesting for biotechnological applications, with ...
Lennartsson, Patrik R.,, Mohammadi, Marzieh,, Asadollahzadeh, Mohammadtaghi,   +2 more
core   +1 more source