STAR ProtocolsSummary: Phosphatidylinositol 3-phosphate (PI3P) levels govern membrane trafficking in Candida glabrata. Here, we present a confocal imaging-based protocol for PI3P localization analysis using the GFP-FYVE (found in Fab1, YOTB, Vac1, and EEA1) fusion ...
Fizza Askari, Rupinder Kaur
doaj +1 more source
Aspartyl proteases target host actin nucleator complex protein to limit epithelial innate immunity
EMBO ReportsEpithelial-immune cell communication is pivotal to control microbial infections. We show that glycosylphosphatidylinositol-linked aspartyl proteases (Yapsins) of the human opportunistic pathogenic yeast Candida glabrata (Cg) thwart epithelial cell (EC ...
Sandip Patra, Rupinder Kaur
doaj +1 more source
Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva
SensorsBiosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting ...
Paweł Wityk +6 more
doaj +1 more source
The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery
Frontiers in Microbiology, 2019 Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodology to extract microbial DNA, since biases introduced ...
Kristýna Fiedorová +12 more
doaj +1 more source
Sydowia polyspora dominates fungal communities carried by two Tomicus species in pine plantations threatened by Fusarium circinatum [PDF]
, 2017 Producción CientíficaBark beetles (Coleoptera, Scolytinae) carry a diverse filamentous fungal community sometimes acting as vectors or carriers of phytopathogens.
Díez Casero, Julio Javier +6 more
core +2 more sources
Identification of some human pathogenic fungi using four DNA extraction methods
Journal of Applied Sciences and Environmental Management, 2017 Dermatophytes being animal and human pathogenic fungi infect some human at one point or the other in their lifetime. For effective control of dermatophytes, accurate identification of the specific species/strain involved must be known.
TO Samuel, AM Ebabhi, AA Adekunle
doaj +1 more source
Uncovering unseen fungal diversity from plant DNA banks [PDF]
PeerJ, 2017 Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve ...
Erin M. Datlof +7 more
doaj +2 more sources
An RNAi-Based Control of Fusarium graminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and Is Controlled by the Fungal Silencing Machinery [PDF]
, 2016 Meeting the increasing food and energy demands of a growing population will require the development of ground-breaking strategies that promote sustainable plant production.
Abdellatef, Eltayb +13 more
core +3 more sources
Frontiers in Plant Science, 2023 Plant pathogenic fungi cause severe yield losses and mycotoxin contamination in crops. The precise and rapid detection of fungal pathogens is essential for effective disease management. Sequencing universal DNA barcodes has become the standard method for
Hosung Jeon +6 more
doaj +1 more source
Pyrosequencing analysis of fungal assemblages from geographically distant, disparate soils reveals spatial patterning and a core mycobiome [PDF]
, 2013 Identifying a soil core microbiome is crucial to appreciate the established microbial consortium, which is not usually subjected to change and, hence, possibly resistant/resilient to disturbances and a varying soil context. Fungi are a major part of soil
Bagella, Simonetta +13 more
core +5 more sources