Results 1 to 10 of about 2,856,737 (303)

Fast preparation of fungal DNA for PCR screening

Journal of Microbiological Methods, 2011
Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate DNA templates from yeast and filamentous fungal materials for PCR. This method may be widely applicable to diverse fungal species in clinical and basic studies.
Kuang-Hung, Liu, Yu-Ling, Yeh, Wei-Chiang, Shen   +2 more
openaire   +2 more sources

Application of cell‐free DNA fungal polymerase chain reaction for invasive fungal disease evaluation in pediatric oncology and stem cell transplant patients

Pediatric Blood & Cancer
Molecular diagnostics may enable early, noninvasive detection of invasive fungal disease (IFD) in immunocompromised patients. Cell‐free deoxyribonucleic acid (cfDNA) fungal polymerase chain reaction (PCR) assays were recently incorporated into ...
L. E. Kushner, H. Schwenk, FeiFei Qin, Derek Boothroyd, Catherine Aftandilian   +4 more
semanticscholar   +1 more source

Quantitative Analysis of Fungal DNA in Chronic Rhinosinusitis

The Laryngoscope, 2004
AbstractObjectives/Hypothesis Fungi have been recognized as important pathogens in sinusitis; however, they are equally present in patients with and without sinusitis. The authors postulated that the quantity of fungal DNA in the nose is determinant of disease, is greater in patients with chronic rhinosinusitis, and is directly correlated to their ...
Michael C, Scheuller, Andrew H, Murr, Andrew N, Goldberg, Anand N, Mhatre, Anil K, Lalwani   +4 more
openaire   +2 more sources

Polyamines, DNA Methylation, and Fungal Differentiation

Critical Reviews in Microbiology, 1994
Mucorales constitute a group of fungi that, because of their growth characteristics, have been used extensively in the study of cell differentiation, cell morphogenesis, and stimuli perception. We have studied the role of polyamine metabolism in the development of different Mucorales, with emphasis on Mucor and Phycomyces species.
openaire   +2 more sources

Tracking Plant, Fungal, and Bacterial DNA in Honey Specimens*

Journal of Forensic Sciences, 2011
Abstract:  Consuming honey can result in adverse effects owing to poisoning by bacterial (botulism) or plant toxins. We have devised a method to extract polymerase chain reaction (PCR) amplifiable DNA of up to c. 400 bp in length based on dialysis of a 15‐mL honey sample for 18 h against deionized water followed by sequential extraction using phenol ...
OLIVIERI, CRISTINA, MAROTA, Isolina, ROLLO, Franco Ugo, LUCIANI, Stefania   +3 more
openaire   +3 more sources

Profiling of bacterial and fungal communities of Mexican cheeses by high throughput DNA sequencing.

Food Research International, 2018
Cheese is a live food whose preparation involves procedures and microbial communities playing an important role for the final product. We characterized the bacterial and fungal diversity of seventeen different Mexican cheeses by high-throughput DNA ...
S. Murugesan, María Paulina Reyes-Mata, Khemlal Nirmalkar, Alejandra Chávez-Carbajal, Josué Isaac Juárez-Hernández, Rosario Erea Torres-Gómez, A. Piña-Escobedo, Otoniel Maya, C. Hoyo‐Vadillo, E. Ramos-Ramírez, J. A. Salazar-Montoya, J. García-Mena   +11 more
semanticscholar   +1 more source

Fungal Nails? DNA Facts Challenge Dystrophic Etiology

Journal of the American Podiatric Medical Association, 2021
Abstract Historically recalcitrant to treatment, infection of the nail unit is a pervasive clinical condition affecting about 10%-20% of the U.S. population; patients present with both cosmetic symptomatology and pain, with subsequent dystrophic morphology.
Beth, Pearce, Stephanie C, Bishop, Curtis E, Jones, John P, Kennedy   +3 more
openaire   +2 more sources

Endophytic fungal DNA, the source of contamination in spruce needle DNA

Molecular Ecology, 1997
DNA isolated and amplified from higher plants may originate from symbiotic microbes occupying plant tissues. A recent report on the phylogeny of Picea contained sequence data that upon later analysis proved to originate from filamentous ascomycetes. Isolates of endophytic fungi from Picea foliage collected from the same location as the original samples
F. J. Camacho, D. S. Gernandt, A. Liston, J. K. Stone, A. S. Klein   +4 more
openaire   +1 more source

Multipartite structure of mitochondrial DNA in a fungal longlife mutant

Plasmid, 1989
Rearrangements in the mitochondrial genome of the longlife mutant ex1 of the ascomycete Podospora anserina have led to a heterogeneous population of subgenomic molecules. The restriction maps of individual subcircles were established using overlapping recombinant lambda phages isolated from an ex 1 mtDNA/EMBL3 library.
E, Schulte, U, Kück, K, Esser
openaire   +2 more sources

Bioadhesive nanoparticles of fungal chitosan for oral DNA delivery

International Journal of Pharmaceutics, 2010
Chitosan is an ideal candidate for oral DNA delivery due to its mucoadhesive properties. Chitosan (CS) produced under GMP conditions from fungal source was used to encapsulate a plasmid DNA coding for a reporter gene. Nanoparticles made by complex coacervation of CS and DNA had a size around 200 nm, a positive zeta potential, a high association of DNA ...
Laurence, Plapied, Gaëlle, Vandermeulen, Benoît, Vroman, Véronique, Préat, Anne, des Rieux   +4 more
openaire   +2 more sources