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Diversity of Fungal DNA Methyltransferases and Their Association With DNA Methylation Patterns [PDF]

open access: yesFrontiers in Microbiology, 2021
DNA methyltransferases (DNMTs) are a group of proteins that catalyze DNA methylation by transferring a methyl group to DNA. The genetic variation in DNMTs results in differential DNA methylation patterns associated with various biological processes.
Yu-Shin Nai   +6 more
doaj   +3 more sources

Comparison of methods for isolating fungal DNA

open access: yesPractical Laboratory Medicine, 2021
Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano   +7 more
doaj   +4 more sources

The Pattern and Function of DNA Methylation in Fungal Plant Pathogens [PDF]

open access: yesMicroorganisms, 2020
To successfully infect plants and trigger disease, fungal plant pathogens use various strategies that are dependent on characteristics of their biology and genomes.
Chang He   +3 more
doaj   +3 more sources

Comparison of methods for isolating fungal DNA [PDF]

open access: yesCzech Journal of Food Sciences, 2011
In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj   +2 more sources

Influence of DNA extraction kits on freshwater fungal DNA metabarcoding [PDF]

open access: yesPeerJ, 2021
Abstract Background Environmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested.
Shunsuke Matsuoka   +3 more
openaire   +3 more sources

Epigenetic Regulation of Antifungal Drug Resistance

open access: yesJournal of Fungi, 2022
In medical mycology, epigenetic mechanisms are emerging as key regulators of multiple aspects of fungal biology ranging from development, phenotypic and morphological plasticity to antifungal drug resistance.
Sandip Patra   +3 more
doaj   +1 more source

An Assay to Determine NAD(P)H: Quinone Oxidoreductase Activity in Cell Extracts from Candida glabrata

open access: yesBio-Protocol, 2021
Flavodoxin-like proteins (Fld-LPs) are an important constituent of the oxidative stress defense system in several organisms and highly conserved from bacteria to humans.
Anamika Battu   +2 more
doaj   +1 more source

Phosphatidylinositol 3-phosphate regulates iron transport via PI3P-binding CgPil1 protein

open access: yesCell Reports, 2023
Summary: Iron homeostasis, which is pivotal to virulence, is regulated by the phosphatidylinositol 3-kinase CgVps34 in the human fungal pathogen Candida glabrata.
Fizza Askari   +2 more
doaj   +1 more source

SUMOylation in Human Pathogenic Fungi: Role in Physiology and Virulence

open access: yesJournal of Fungi, 2020
The small ubiquitin-related modifier (SUMO) protein is an important component of the post-translational protein modification systems in eukaryotic cells.
Mahima Sagar Sahu   +3 more
doaj   +1 more source

Detailed Protocol to Perform Direct PCR Using Filamentous Fungal Biomass—Tips and Considerations

open access: yesBio-Protocol, 2023
The precise and rapid detection of fungi is important in various fields, including clinics, industry, and agriculture. While sequencing universal DNA barcodes remains the standard method for species identification and phylogenetic analysis, a significant
Hosung Jeon, Hokyoung Son, Kyunghun Min
doaj   +1 more source

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