Results 31 to 40 of about 2,149,898 (257)

Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?

open access: yesPLoS ONE, 2020
BACKGROUND:High-throughput sequencing techniques are used to analyse the diversity of the respiratory microbiota in health and disease. Although extensive data are available regarding bacterial respiratory microbiota, its fungal component remains poorly ...
Cécile Angebault   +4 more
doaj   +1 more source

General Fungal DNA Extraction v2

open access: yes, 2023
This is a routine protocol for extracting DNA from various fungi. This extraction method is suitable for follow-up molecular work such as PCR amplification.
Angie Macias   +2 more
openaire   +1 more source

Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation [PDF]

open access: yes, 2019
Species identification lies at the heart of biodiversity studies that has in recent years favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source for diverse and high-quality reference materials in microbiology, and yet the
Vries, M., de   +30 more
core   +1 more source

Fairy rings harbor distinct soil fungal communities and high fungal diversity in a montane grassland

open access: yes, 2020
Increasing numbers of fungal species have been described recently from semi-natural grassland soils, raising the conservation interest of these species-rich habitats. Here, we characterize the soil fungal community inhabiting six fairy rings in a montane
Sveriges lantbruksuniversitet.   +1 more
core   +1 more source

Fungal CSL transcription factors [PDF]

open access: yes, 2007
Background: The CSL (CBF1/RBP-J kappa/Suppressor of Hairless/LAG-1) transcription factor family members are well-known components of the transmembrane receptor Notch signaling pathway, which plays a critical role in metazoan development. They function as
Prevorovsky, M   +8 more
core   +1 more source

Fungal CTAB DNA Extraction v1 [PDF]

open access: yes, 2020
This protocol is a CTAB DNA extraction method for filamentous fungi. Its purpose is to extract high molecular weight genomic DNA for genome sequencing.
Derreck Carter-House   +3 more
openaire   +1 more source

ITS2 REGION-BASED MOLECULAR IDENTIFICATION OF FUNGAL PATHOGENS IN EQUINE CORNEAL ULCERATION [PDF]

open access: yesAssiut Veterinary Medical Journal, 2014
This study aimed to assess the utility of polymerase chain reaction (PCR) and DNA sequencing in diagnosing fungal ulcerative keratitis in horses and compare their sensitivity with conventional microbiologic techniques used in laboratories.
M. HAMED   +3 more
doaj   +1 more source

Mononucleotide repeats are asymmetrically distributed in fungal genes [PDF]

open access: yes, 2008
Background Systematic analyses of sequence features have resulted in a better characterisation of the organisation of the genome. A previous study in prokaryotes on the distribution of sequence repeats, which are notoriously variable and can disrupt the ...
Graaff, L.H., de   +3 more
core   +1 more source

Using DNA Barcoding in Fungal Taxonomy

open access: yesYüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2020
Totally, 1.5 million fungal species estimated, of which less than 10 percent have been described to date. Recent advances in molecular biology as well as gene sequencing technologies facilitate the discovery and identification of new species in various groups of living organisms, especially fungi.
Younes Rezaee Danesh, Semra Demir
openaire   +3 more sources

Real-time quantitative PCR for analysis of candidate fungal biopesticides against malaria: Technique validation and first applications [PDF]

open access: yes, 2009
Recent research has indicated that fungal biopesticides could augment existing malaria vector control tools. Here we present a set of methodologies to monitor the in vivo kinetics of entomopathogenic fungi in Anopheles in the presence or absence of ...
Thomas, Matthew B.   +9 more
core   +1 more source

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