Results 31 to 40 of about 2,856,737 (303)
Rapid Mini-Preparation of Fungal DNA for PCR [PDF]
Journal of Clinical Microbiology, 2000 Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Although PCR amplification can be performed directly for various microbial cultures, for filamentous fungi and yeasts, prior isolation of DNA is often preferred.
D, Liu, S, Coloe, R, Baird, J, Pederson
openaire +2 more sources
Molecular Ecology Resources, 2020 DNA metabarcoding has become a powerful approach for analysing complex communities from environmental samples, but there are still methodological challenges limiting its full potential. While conserved DNA markers, like 16S and 18S, often are not able to
Eva Lena F Estensmo +5 more
semanticscholar +1 more source
A simple method for extraction of fungal genomic DNA [PDF]
Letters in Applied Microbiology, 2000 We have developed a new, simple and effective method for extraction of fungal genomic DNA. The initial steps involved suspension of freeze-dried mycelium in buffer containing sodium dodecyl sulphate, detachment of DNA from polysaccharides by mild shearing, NaCl precipitation of polysaccharides and protein, chloroform extraction and ethanol ...
T H, Al-Samarrai, J, Schmid
openaire +2 more sources
Candida glabrata: A Lot More Than Meets the Eye
Microorganisms, 2019 Candida glabrata is an opportunistic human fungal pathogen that causes superficial mucosal and life-threatening bloodstream infections in individuals with a compromised immune system. Evolutionarily, it is closer to the non-pathogenic yeast Saccharomyces
Kundan Kumar +3 more
doaj +1 more source
Use of magnetic beads to extract fungal DNA [PDF]
Mycoses, 2005 SummaryAuthors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum, Trichophyton rubrum, T. interdigitale, T.
FAGGI, ELISABETTA +2 more
openaire +3 more sources
Mycelial DNA persistence in a forest soil
Environmental DNA, 2021 Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering ...
Matthew Gordon, Kelli Van Norman
doaj +1 more source
Identification of Contaminating Fungal DNA Sequences in Zymolyase [PDF]
Journal of Clinical Microbiology, 1999 ABSTRACT When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu ...
D, Rimek, A P, Garg, W H, Haas, R, Kappe
openaire +2 more sources
Evolutionary Relationship and the Sequence Similarities among Different Fungal Species Infecting Birds Captured from Different Areas in Denmark [PDF]
Archives of Razi Institute, 2022 Fungal diseases are the common cause of death in wild animals and birds of prey. This study was designed to investigate the development of fungal infections among wild birds in Denmark.
Z Shaker Al-Rubaiee +2 more
doaj +1 more source
Studies in Mycology, 2018 Species identification lies at the heart of biodiversity studies that has in recent years favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source for diverse and high-quality reference materials in microbiology, and yet the
D. Vu +14 more
semanticscholar +1 more source
mBio, 2021 Although relatively few in number, studies of DNA methylation have shown that fungal DNA methylation is implicated in development, genome integrity, and genome defense.
Yo-Han Ko +3 more
doaj +1 more source