Results 51 to 60 of about 2,856,737 (303)

Evaluation of Oral Cavity DNA Extraction Methods on Bacterial and Fungal Microbiota

Scientific Reports, 2019
The objective of this study was to evaluate the most effective method of DNA extraction of oral mouthwash samples for use in microbiome studies that utilize next generation sequencing (NGS).
J. Rosenbaum, Mykhaylo Usyk, Zigui Chen, Christine P. Zolnik, H. Jones, L. Waldron, J. Dowd, L. Thorpe, R. Burk   +8 more
semanticscholar   +1 more source

The imperial coach of D. Pedro II: DNA fungal identification in selected materials and its relation to biodeterioration and aerobiology

, 2013
A berlinda de aparato do imperador D. Pedro II foi construida pela companhia britânica Pearce & Countz, responsavel pela construcao das carruagens da monarquia britânica, para sua cerimonia de coroacao, em 1841.
M. Lutterbach, A. Oliveira, E. M. Zanatta, A. C. T. D. Costa   +3 more
semanticscholar   +1 more source

Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications

PLoS ONE, 2018
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered by poor DNA quality and purity, particularly in challenging plant species, rich in secondary metabolites.
P. Inglis, M. Pappas, L. V. Resende, D. Grattapaglia   +3 more
semanticscholar   +1 more source

Database establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α) 1.

Genome, 2019
With new or emerging fungal infections, human and animal fungal pathogens are a growing threat worldwide. Current diagnostic tools are slow, non-specific at the species and subspecies levels, and require specific morphological expertise to accurately ...
W. Meyer, L. Irinyi, Minh Thuy Vi Hoang, V. Robert, D. Garcia-Hermoso, M. Desnos-Ollivier, Chompoonek Yurayart, Chi-Ching Tsang, Chun-Yi Lee, P. Woo, I. Pchelin, S. Uhrlass, P. Nenoff, A. Chindamporn, S. Chen, P. Hebert, T. Sorrell   +16 more
semanticscholar   +1 more source

Pan-cancer analyses reveal cancer-type-specific fungal ecologies and bacteriome interactions

Cell, 2022
Summary Cancer-microbe associations have been explored for centuries, but cancer-associated fungi have rarely been examined. Here, we comprehensively characterize the cancer mycobiome within 17,401 patient tissue, blood, and plasma samples across 35 ...
Lian Narunsky-Haziza, G. Sepich-Poore, Ilana Livyatan, Omer Asraf, C. Martino, D. Nejman, N. Gavert, J. Stajich, Guy Amit, Antonio González, S. Wandro, G. Perry, R. Ariel, A. Meltser, Justin P. Shaffer, Qiyun Zhu, Nora Balint-Lahat, I. Barshack, M. Dadiani, E. Gal-Yam, S. Patel, A. Bashan, Austin D. Swafford, Yitzhak Pilpel, R. Knight, R. Straussman   +25 more
semanticscholar   +1 more source

The Effectiveness of PCR in Diagnosis of Fungal Keratitis

Oftalʹmologiâ, 2020
Fungi implicated in mycotic keratitis include different species. Conventional methods for the diagnosis of fungal keratitis include staining of corneal scarpings, culture medium (Sabouraud agar) for isolating fungi.Purpose.
G. I. Krichevskaya, L. A. Kovaleva, I. D. Zyurnyayeva, P. V. Makarov, A. E. Andryushin   +4 more
doaj   +1 more source

Evaluation of the ribosomal DNA internal transcribed spacer (ITS), specifically ITS1 and ITS2, for the analysis of fungal diversity by deep sequencing

PLoS ONE, 2018
The nuclear ribosomal DNA internal transcribed spacer (ITS) has been widely used to assess the fungal composition in different environments by deep sequencing.
Rui-Heng Yang, Jin-He Su, Junjun Shang, Yingying Wu, Yan Li, D. Bao, Yijian Yao   +6 more
semanticscholar   +1 more source

Using DNA Barcoding in Fungal Taxonomy

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2020
Totally, 1.5 million fungal species estimated, of which less than 10 percent have been described to date. Recent advances in molecular biology as well as gene sequencing technologies facilitate the discovery and identification of new species in various groups of living organisms, especially fungi.
Younes Rezaee Danesh, Semra Demir
openaire   +3 more sources

Fungal CTAB DNA Extraction v1 [PDF]

, 2020
This protocol is a CTAB DNA extraction method for filamentous fungi. Its purpose is to extract high molecular weight genomic DNA for genome sequencing.
Derreck Carter-House, Jason E Stajich, Sarah Unruh, Tania Kurbessoian   +3 more
openaire   +1 more source

An overview of using fungal DNA for the diagnosis of invasive mycoses

Expert Review of Molecular Diagnostics, 2022
Fungal PCR has undergone considerable standardization and, together with the availability of commercial assays, external quality assessment schemes, and extensive performance validation data, is ready for widespread use for the screening and diagnosis of invasive fungal disease (IFD).Drawing on the experience and knowledge of the leads of the various ...
White, P Lewis, Alanio, Alexandre, Brown, Lottie, Cruciani, Mario, Hagen, Ferry, Gorton, Rebecca, Lackner, Michaela, Millon, Laurence, Morton, C Oliver, Rautemaa-Richardson, Riina, Barnes, Rosemary, Donnelly, J Peter, Loffler, Juergen   +12 more
openaire   +6 more sources