Results 71 to 80 of about 2,149,898 (257)

Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimens

mSystems
Long-read metagenomics provides a promising alternative approach to fungal identification, circumventing methodological biases, associated with DNA amplification, which is a prerequisite for DNA barcoding/metabarcoding based on the primary fungal DNA ...
Nattapong Langsiri, Wieland Meyer, Laszlo Irinyi, Navaporn Worasilchai, Nuttapon Pombubpa, Thidathip Wongsurawat, Piroon Jenjaroenpun, J. Jennifer Luangsa-ard, Ariya Chindamporn   +8 more
doaj   +1 more source

Repurposing FDA‐Approved Drugs to Inhibit Fungal PPTases for Broad‐Spectrum Synergy

Advanced Science, EarlyView.
Fungal secondary metabolites serve as crucial virulence effectors; however, the potential of their biosynthetic pathways as antifungal targets remains inadequately comprehended. Repurposed FDA‐approved drugs inhibit fungal PPTase (essential for secondary metabolism) in vivo.
Zili Song, Can Zhao, Qiaoling Wang, Hongjiao Zhang, Xiao Liu, Linqi Wang, Michael Bromley, Wen‐Bing Yin   +7 more
wiley   +1 more source

Optimal liquid-based DNA preservation for DNA barcoding of field-collected fungal specimens

Heliyon
Preserving fungal tissue DNA in the field is essential for molecular ecological research, enabling the study of fungal biodiversity and community dynamics. This study systematically compares two liquid-based preservation solutions, RNAlater and DESS, for
Yu-Ja Lee, Guan Jie Phang, Che-Chih Chen, Jie-Hao Ou, Yu-Hsuan Fan, Yin-Tse Huang   +5 more
doaj   +1 more source

Functional–Taxonomic Scaling Resolves Conflicting Average Genome Size Estimates Across Environmental Gradients

Advanced Science, EarlyView.
Based on genome size‐functional breadth theory, we introduce and validate a framework that adjudicates conflicting average genome size (AGS) estimates by testing which estimate yields a gap between scaled functional diversity and scaled taxonomic diversity that is consistent with the expected positive association between genome size and gene functional
Huanhuan Zhu, Xiaoye Chen, Qiushi Li, Chenyu Sun, Haihua Xia, Yingxin Huang, Cong Wang, Peilin Chen, Cheng Gao   +8 more
wiley   +1 more source

Primer pairs, PCR conditions, and peptide nucleic acid clamps affect fungal diversity assessment from plant root tissues

Mycology
High-throughput sequencing has become a prominent tool to assess plant-associated microbial diversity. Still, some technical challenges remain in characterising these communities, notably due to plant and fungal DNA co-amplification.
Chloé Viotti, Michel Chalot, Peter G. Kennedy, François Maillard, Sylvain Santoni, Damien Blaudez, Coralie Bertheau   +6 more
doaj   +1 more source

The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles

Methods and Protocols, 2020
Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in
Anna Ojo-Okunola, Shantelle Claassen-Weitz, Kilaza S. Mwaikono, Sugnet Gardner-Lubbe, Heather J. Zar, Mark P. Nicol, Elloise du Toit   +6 more
doaj   +1 more source

Stage-specific DNA methylation in a fungal plant pathogen [PDF]

Journal of Bacteriology, 1986
Significantly more 5-methylcytosine residues were found in the DNA from the dormant sclerotia of Phymatotrichum omnivorum than in the DNA from the metabolically active mycelia of the fungus, as shown by high-pressure liquid chromatography of acid-hydrolyzed DNA digests and by restriction of the DNA with the isoschizomers MspI and HpaII.
E R, Jupe, J M, Magill, C W, Magill
openaire   +2 more sources

Sex‐ and Time‐Resolved Physiological and Gut Ecosystem Responses to a Food‐Grade Microbiome‐Targeted Intervention in Collegiate Endurance Athletes

Advanced Science, EarlyView.
In collegiate endurance athletes, a 6‐week randomized CPYE intervention yielded time‐ and sex‐dependent shifts in field‐based cardiorespiratory, metabolic, and redox indices, accompanied by changes in gut multi‐omics profiles. An exploratory extension during intensified training generates hypotheses warranting future validation.
Meng Zhang, Le Ge, Kaiyang Liu, Yanrong Liu, Guolin Zhao, Lai‐Yu Kwok, Zheng Sun, Zhihong Sun, Wenyi Zhang   +8 more
wiley   +1 more source

The primer fITS9 prevents chimera formation during fungal DNA amplification in a bark beetle DNA background

, 2015
Insects are frequently associated with fungi in natural habitats. Molecular detection of fungal species using the primer pair ITS1F and ITS4 to amplify the ribosomal ITS region has become standard for studies of fungal ecology. When addressing insect and
Ihrmark, Katarina,, Stenlid, Jan,, Strid, Ylva,, Sveriges lantbruksuniversitet., Swedish University of Agricultural Sciences.   +4 more
core   +1 more source

Uncovering unseen fungal diversity from plant DNA banks [PDF]

PeerJ, 2017
Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve ...
Erin M. Datlof, Anthony S. Amend, Kamala Earl, Jeremy Hayward, Clifford W. Morden, Rachael Wade, Geoffrey Zahn, Nicole A. Hynson   +7 more
doaj   +2 more sources