Results 71 to 80 of about 1,916,213 (308)

Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimens

open access: yesmSystems
Long-read metagenomics provides a promising alternative approach to fungal identification, circumventing methodological biases, associated with DNA amplification, which is a prerequisite for DNA barcoding/metabarcoding based on the primary fungal DNA ...
Nattapong Langsiri   +8 more
doaj   +1 more source

The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles

open access: yesMethods and Protocols, 2020
Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in
Anna Ojo-Okunola   +6 more
doaj   +1 more source

Calcineurin‐Dependent Stress Adaptation Enables Caspofungin Heteroresistance Leading to Stable Resistance in Candida Glabrata

open access: yesAdvanced Science, EarlyView.
Caspofungin heteroresistance is prevalent in clinical Candida glabrata isolates and depends on calcineurin‐mediated stress adaptation. This transient phenotype serves as a reservoir for resistance evolution, enabling the emergence of stable resistant descendants under prolonged drug pressure.
Yanyu Su   +7 more
wiley   +1 more source

Optimal liquid-based DNA preservation for DNA barcoding of field-collected fungal specimens

open access: yesHeliyon
Preserving fungal tissue DNA in the field is essential for molecular ecological research, enabling the study of fungal biodiversity and community dynamics. This study systematically compares two liquid-based preservation solutions, RNAlater and DESS, for
Yu-Ja Lee   +5 more
doaj   +1 more source

Uncovering unseen fungal diversity from plant DNA banks [PDF]

open access: yesPeerJ, 2017
Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve ...
Erin M. Datlof   +7 more
doaj   +2 more sources

Application of direct PCR for phylogenetic analysis of Fusarium fujikuroi species complex isolated from rice seeds

open access: yesFrontiers in Plant Science, 2023
Plant pathogenic fungi cause severe yield losses and mycotoxin contamination in crops. The precise and rapid detection of fungal pathogens is essential for effective disease management. Sequencing universal DNA barcodes has become the standard method for
Hosung Jeon   +6 more
doaj   +1 more source

A Comprehensive Microbial Gene Catalog of the Human Airway Microbiome Across Anatomical Sites and Geographic Regions

open access: yesAdvanced Science, EarlyView.
An integrated Human Airway Microbiome Gene Catalog (iHAMGC) is constructed from 12,273 metagenomic airway samples, providing a high‐resolution reference of respiratory microbial genes. The catalog enables systematic exploration of taxonomic and functional variation across airway niches and geographic regions, revealing site‐specific and region ...
Qing Zhang   +15 more
wiley   +1 more source

Ectomycorrhizal fungi - Molecular tools to study species and functional diversity [PDF]

open access: yes, 2003
The extramatrical mycelium of ectomycorrhizal (EM) fungi represents a significant component of the ectomycorrhizal (EM) symbiosis, as the mycelium is primarily involved in the uptake of water and nutrients from the soil.
Landeweert, R.
core  

Primer pairs, PCR conditions, and peptide nucleic acid clamps affect fungal diversity assessment from plant root tissues

open access: yesMycology
High-throughput sequencing has become a prominent tool to assess plant-associated microbial diversity. Still, some technical challenges remain in characterising these communities, notably due to plant and fungal DNA co-amplification.
Chloé Viotti   +6 more
doaj   +1 more source

Subversion of Atypical Mucin Traps by a Spore‐Coat Effector Blocks Cellular Immunity in Drosophila

open access: yesAdvanced Science, EarlyView.
While the entomopathogenic fungus Metarhizium robertsii can evade insect humoral immunity, its subversion of cellular defenses is still elusive. We identify Eac1 as a spore‐coat protein essential for infecting drosophilids by targeting atypical mucins (Sgf1 and Sgf2), which can entrap spores in a gel matrix and thereby facilitate hemocyte encapsulation.
Shiqin Li   +7 more
wiley   +1 more source

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