Results 81 to 90 of about 2,856,737 (303)
Fungal Genetics and Biology, 2018 Current challenges in the study and biotechnological exploitation of filamentous fungi are the optimization of DNA cloning and fungal genetic transformation beyond model fungi, the open exchange of ready-to-use and standardized genetic elements among the
Miguel Hernanz-Koers +6 more
semanticscholar +1 more source
Advanced Science, EarlyView.An iron‐based nanozyme selectively eliminates intratumoral P. anaerobius while catalytically generating ROS to induce ferroptosis, synergistically suppressing colorectal cancer growth and activating anti‐tumor immunity through immunogenic cell death. ABSTRACT The intratumoral microbiota is a critical determinant of therapeutic outcomes in colorectal ...
Yinghao Cao +11 more
wiley +1 more source
Frontiers in Plant Science, 2023 Plant pathogenic fungi cause severe yield losses and mycotoxin contamination in crops. The precise and rapid detection of fungal pathogens is essential for effective disease management. Sequencing universal DNA barcodes has become the standard method for
Hosung Jeon +6 more
doaj +1 more source
PLoS ONE, 2018 Background The human intestinal microbiota contains a vast community of microorganisms increasingly studied using high-throughput DNA sequencing. Standardized protocols for storage and DNA extraction from fecal samples have been established mostly for ...
C. Angebault +5 more
semanticscholar +1 more source
Dissecting the Ecological Structure of Health and Disease in the Global Gut Microbiome
Advanced Science, EarlyView.We introduce Wiredancer, a framework that identifies three continuous ecological factors of the gut microbiota. These factors exhibit distinct patterns across health and disease, jointly capturing disrupted ecological stability and offering a new perspective for precision diagnostics and therapeutic strategies.
Baoyuan Zhu +19 more
wiley +1 more source
mSystemsLong-read metagenomics provides a promising alternative approach to fungal identification, circumventing methodological biases, associated with DNA amplification, which is a prerequisite for DNA barcoding/metabarcoding based on the primary fungal DNA ...
Nattapong Langsiri +8 more
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The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles
Methods and Protocols, 2020 Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in
Anna Ojo-Okunola +6 more
doaj +1 more source
Evaluation of three DNA extraction methods from fungal cultures.
Medical Journal Armed Forces India, 2017 Polymerase chain reaction (PCR) based assays have been developed to amplify DNA of fungal pathogens as culture-based detection methods show low sensitivity. In order to perform a sensitive, specific, and reliable PCR based assay, the availability of pure
Mahadevan Kumar, M. Mugunthan
semanticscholar +1 more source
Connecting the multiple dimensions of global soil fungal diversity
Science Advances, 2023 How the multiple facets of soil fungal diversity vary worldwide remains virtually unknown, hindering the management of this essential species-rich group.
V. Mikryukov +105 more
semanticscholar +1 more source
Advanced Science, EarlyView.ABSTRACT Gut microbiota dysbiosis promotes colorectal cancer (CRC) tumorigenesis. A global fecal metagenomic analysis identified Gemella morbillorum as a key contributor to the CRC‐associated microbiota. Fluorescence in situ hybridization revealed that Gemella morbillorum is enriched in CRC tumor tissues compared to adjacent normal tissues.
Zhen Wang +8 more
wiley +1 more source