Tracking fungal community responses to maize plants by DNA- and RNA-based pyrosequencing. [PDF]
PLoS ONE, 2013 We assessed soil fungal diversity and community structure at two sampling times (t1 = 47 days and t2 = 104 days of plant age) in pots associated with four maize cultivars, including two genetically modified (GM) cultivars by high-throughput ...
Eiko E Kuramae +6 more
doaj +1 more source
DNA quantification of basidiomycetous fungi during storage of logging residues [PDF]
PeerJ, 2015 The demand for bioenergy caused an increased use of logging residues, branches and treetops that were previously left on the ground after harvesting. Residues are stored outdoors in piles and it is unclear to what extent fungi transform this material ...
Isabella Børja +3 more
doaj +2 more sources
Introduction of a bead beating step improves fungal DNA extraction from selected patient specimens
International Journal of Medical Microbiology, 2020 In immunocompromised patients a colonisation with fungi carries the risk to develop serious invasive fungal infection. An early detection is therefore important, but not optimal hitherto.
Sebastian Scharf +5 more
doaj +1 more source
Molecular Characterization of Subunit G of the Vacuolar ATPase in Pathogen Dermatophyte Trichophyton rubrum [PDF]
Iranian Journal of Public Health, 2006 Trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. Several properties of this fungus have been investigated so far.
S Rezaie +3 more
doaj +1 more source
PLoS ONE, 2020 BACKGROUND:High-throughput sequencing techniques are used to analyse the diversity of the respiratory microbiota in health and disease. Although extensive data are available regarding bacterial respiratory microbiota, its fungal component remains poorly ...
Cécile Angebault +4 more
doaj +1 more source
Comparison of methods for isolating fungal DNA
Practical Laboratory Medicine, 2021 Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano +7 more
doaj +1 more source
ITS2 REGION-BASED MOLECULAR IDENTIFICATION OF FUNGAL PATHOGENS IN EQUINE CORNEAL ULCERATION [PDF]
Assiut Veterinary Medical Journal, 2014 This study aimed to assess the utility of polymerase chain reaction (PCR) and DNA sequencing in diagnosing fungal ulcerative keratitis in horses and compare their sensitivity with conventional microbiologic techniques used in laboratories.
M. HAMED +3 more
doaj +1 more source
The Pattern and Function of DNA Methylation in Fungal Plant Pathogens
Microorganisms, 2020 To successfully infect plants and trigger disease, fungal plant pathogens use various strategies that are dependent on characteristics of their biology and genomes.
Chang He +3 more
doaj +1 more source
The Effectiveness of PCR in Diagnosis of Fungal Keratitis
Oftalʹmologiâ, 2020 Fungi implicated in mycotic keratitis include different species. Conventional methods for the diagnosis of fungal keratitis include staining of corneal scarpings, culture medium (Sabouraud agar) for isolating fungi.Purpose.
G. I. Krichevskaya +4 more
doaj +1 more source
Spatial structure of fungal DNA assemblages revealed with eDNA metabarcoding in a forest river network in western Japan [PDF]
Metabarcoding and Metagenomics, 2019 Growing evidence has revealed high diversity and spatial heterogeneity of fungal communities in local habitats of terrestrial ecosystems. Recently, the analysis of environmental DNA has been undertaken to study the biodiversity of organisms, such as ...
Shunsuke Matsuoka +5 more
doaj +3 more sources