Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]
IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
A Lenore Ackerman +8 more
doaj +4 more sources
Dye-based qPCR assays for the separate quantification of total bacterial, fungal, arthropod, and plant DNA. [PDF]
Characterization of bacterial, fungal, arthropod, and plant communities from bulk environmental samples can enhance our understanding of the biodiversity on Earth and aid in biomonitoring of various biomes.
Teresa M Tiedge +2 more
doaj +2 more sources
Generating Phenotypic Diversity in a Fungal Biocatalyst to Investigate Alcohol Stress Tolerance Encountered during Microbial Cellulosic Biofuel Production [PDF]
peer-reviewedConsolidated bioprocessing (CBP) of lignocellulosic biomass offers an alternative route to renewable energy. The crop pathogen Fusarium oxysporum is a promising fungal biocatalyst because of its broad host range and innate ability to co ...
Doohan, Fiona +2 more
core +12 more sources
Epigenetic Regulation of Antifungal Drug Resistance
In medical mycology, epigenetic mechanisms are emerging as key regulators of multiple aspects of fungal biology ranging from development, phenotypic and morphological plasticity to antifungal drug resistance.
Sandip Patra +3 more
doaj +1 more source
Flavodoxin-like proteins (Fld-LPs) are an important constituent of the oxidative stress defense system in several organisms and highly conserved from bacteria to humans.
Anamika Battu +2 more
doaj +1 more source
Phosphatidylinositol 3-phosphate regulates iron transport via PI3P-binding CgPil1 protein
Summary: Iron homeostasis, which is pivotal to virulence, is regulated by the phosphatidylinositol 3-kinase CgVps34 in the human fungal pathogen Candida glabrata.
Fizza Askari +2 more
doaj +1 more source
SUMOylation in Human Pathogenic Fungi: Role in Physiology and Virulence
The small ubiquitin-related modifier (SUMO) protein is an important component of the post-translational protein modification systems in eukaryotic cells.
Mahima Sagar Sahu +3 more
doaj +1 more source
Diversity of Fungal DNA Methyltransferases and Their Association With DNA Methylation Patterns
DNA methyltransferases (DNMTs) are a group of proteins that catalyze DNA methylation by transferring a methyl group to DNA. The genetic variation in DNMTs results in differential DNA methylation patterns associated with various biological processes.
Yu-Shin Nai +6 more
doaj +1 more source
Detailed Protocol to Perform Direct PCR Using Filamentous Fungal Biomass—Tips and Considerations
The precise and rapid detection of fungi is important in various fields, including clinics, industry, and agriculture. While sequencing universal DNA barcodes remains the standard method for species identification and phylogenetic analysis, a significant
Hosung Jeon, Hokyoung Son, Kyunghun Min
doaj +1 more source
Candida glabrata: A Lot More Than Meets the Eye
Candida glabrata is an opportunistic human fungal pathogen that causes superficial mucosal and life-threatening bloodstream infections in individuals with a compromised immune system. Evolutionarily, it is closer to the non-pathogenic yeast Saccharomyces
Kundan Kumar +3 more
doaj +1 more source

