Results 11 to 20 of about 1,916,213 (308)

The Quest for a General and Reliable Fungal DNA Barcode [PDF]

The Open Applied Informatics Journal, 2011
DNA sequences are key elements for both identification and classification of living organisms. Mainly for historical reasons, a limited number of genes are currently used for this purpose. From a mathematical point of view, any DNA segment, at any location, even outside of coding regions and even if they do not align, could be used as long as PCR ...
V. Robert, S. Szöke, U. Eberhardt, CARDINALI, Gianluigi, W. Meyer, K. A. Seifert, C. A. Lévesque, C. T. Lewis   +7 more
openaire   +4 more sources

Rapid Mini-Preparation of Fungal DNA for PCR [PDF]

Journal of Clinical Microbiology, 2000
Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Although PCR amplification can be performed directly for various microbial cultures, for filamentous fungi and yeasts, prior isolation of DNA is often preferred.
D, Liu, S, Coloe, R, Baird, J, Pederson
openaire   +2 more sources

Candida glabrata: A Lot More Than Meets the Eye

Microorganisms, 2019
Candida glabrata is an opportunistic human fungal pathogen that causes superficial mucosal and life-threatening bloodstream infections in individuals with a compromised immune system. Evolutionarily, it is closer to the non-pathogenic yeast Saccharomyces
Kundan Kumar, Fizza Askari, Mahima Sagar Sahu, Rupinder Kaur   +3 more
doaj   +1 more source

A simple method for extraction of fungal genomic DNA [PDF]

Letters in Applied Microbiology, 2000
We have developed a new, simple and effective method for extraction of fungal genomic DNA. The initial steps involved suspension of freeze-dried mycelium in buffer containing sodium dodecyl sulphate, detachment of DNA from polysaccharides by mild shearing, NaCl precipitation of polysaccharides and protein, chloroform extraction and ethanol ...
T H, Al-Samarrai, J, Schmid
openaire   +2 more sources

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Bahkali, A.H., Abd-Elsalam, K.A., Wit, P.J.G.M., de, Moslem, M.A.   +3 more
core   +1 more source

Use of magnetic beads to extract fungal DNA [PDF]

Mycoses, 2005
SummaryAuthors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum, Trichophyton rubrum, T. interdigitale, T.
FAGGI, ELISABETTA, PINI, GABRIELLA, CAMPISI, ENZA   +2 more
openaire   +3 more sources

Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation [PDF]

, 2019
Species identification lies at the heart of biodiversity studies that has in recent years favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source for diverse and high-quality reference materials in microbiology, and yet the
Vries, M., de, de Vries, M, Boekhout, T, A. Al-Hatmi, D. Vu, Groenewald, J.Z., Groenewald, Johannes Zacharias, Gehrmann, T, B. Stielow, de Vries, M., Al-Hatmi, A, Crous, Pedro W., Verkley, G.J.M., Robert, V., Eberhardt, U, G.J.M. Verkley, V. Robert, Houbraken, J, Vu, D, Groenewald, Marizeth, T. Boekhout, J.Z. Groenewald, T. Gehrmann, Gehrmann, T., Al-Hatmi, A., Crous, P.W., Houbraken, J., Eberhardt, U., M. Groenewald, Boekhout, T., U. Eberhardt, Stielow, B., Stielow, B, Robert, Vincent, P.W. Crous, M. de Vries, Vu, D., Groenewald, M., Cardinali, G., G. Cardinali, J. Houbraken   +40 more
core   +2 more sources

Mycelial DNA persistence in a forest soil

Environmental DNA, 2021
Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering ...
Matthew Gordon, Kelli Van Norman
doaj   +1 more source

Identification of Contaminating Fungal DNA Sequences in Zymolyase [PDF]

Journal of Clinical Microbiology, 1999
ABSTRACT When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu ...
D, Rimek, A P, Garg, W H, Haas, R, Kappe
openaire   +2 more sources

RIPCAL: a tool for alignment-based analysis of repeat-induced point mutations in fungal genomic sequences [PDF]

, 2008
Background Repeat-induced point mutation (RIP) is a fungal-specific genome defence mechanism that alters the sequences of repetitive DNA, thereby inactivating coding genes.
Hane, J., Oliver, Richard, James K Hane, Richard P Oliver, Oliver, R.P., Hane James K, Hane, J.K., Oliver Richard P   +7 more
core   +1 more source