Results 81 to 90 of about 6,481,147 (237)

Plant‐produced encapsulin displays non‐typhoidal Salmonella enterica antigens and assembles into mosaic nanoparticles

The FEBS Journal, EarlyView.
This study investigates the potential of encapsulin as an antigen display platform for the development of a candidate Salmonella vaccine for poultry. Encapsulin constructs were transiently expressed in Nicotiana benthamiana where they accumulated to high levels.
Carly A. Charron, Angelo Kaldis, Shabnam Shamriz, Justin B. Renaud, Moussa S. Diarra, Christopher P. Garnham, Rima Menassa   +6 more
wiley   +1 more source

Production and characterization of recombinant pertactin, fimbriae 2 and fimbriae 3 from Bordetella pertussis

BMC Microbiology, 2009
Background Bordetella pertussis is a causative agent of pertussis or whooping cough in humans. Pertactin (Prn), fimbriae 2 (Fim2) and fimbriae 3 (Fim3) of B.
Hou Qiming, Wang Lichan, Wu Lijie, Zhang Huajie, Tan Yajun, Wang Yaying, Xu Yinghua, Zhang Shumin   +7 more
doaj   +1 more source

Orchestrated molecular changes of proliferative, migratory‐fibrillar, synaptic, and postmigratory compartments align with precocious cortex‐type specification in the early human pallium

Journal of Anatomy, EarlyView.
Early human cortical development is organized by transient cellular compartments that define cortical types before mature layers form. Analysis of the human fetal pallium (7.5–15 PCW) shows distinct spatiotemporal trajectories for the archicortex, mesocortex, and neocortex, with delayed but accelerated differentiation in allocortical regions.
Janja Kopić, Fran Božić, Alisa Junaković Munjas, Željka Krsnik, Ivica Kostović   +4 more
wiley   +1 more source

Inflammatory and Immunological Basis of Periodontal Diseases

Journal of Periodontal Research, EarlyView.
The periodontal lesion emerges as an evolving immunological battlefield, where host–microbiome interactions, dysregulated immune responses, fragile resolution mechanisms, and inflammophilic dysbiosis converge to shift the balance from homeostasis to unrestrained tissue destruction.
Giacomo Baima, Marion Arce, Mario Romandini, Thomas Van Dyke   +3 more
wiley   +1 more source

Binding of lactoferrin and free secretory component to enterotoxigenic Escherichia coli [PDF]

, 2001
The ability of two glycoproteins of human milk, lactoferrin and free secretory component, to bind to Escherichia coli colonization factors (CFAs) was investigated using immunocytochemistry assays of enriched fimbrial extracts.
Andréa Nascimento Araújo, Appelmelk, Ashkenazi, Bradford, Brock, Buts, Bühler, Cravioto, Cravioto, Dallas, Erdei, Evans, Evans, Falk, Faruque, Giugliano, Glass, Guth, Inaiara Rosa Oliveira, Kobayashi, Kovar, Laemmli, Loreny Gimenes Giugliano, Mestecky, Mynott, Naidu, Paniagua, Reis, Smyth, Sonia Nair Bao, Towbin, Young   +31 more
core   +1 more source

The Keystone‐Pathogen Hypothesis Updated: The Role of Porphyromonas gingivalis in Periodontitis

Journal of Periodontal Research, EarlyView.
Porphyromonas gingivalis orchestrates a coordinated manipulation of immune and inflammatory responses in periodontal tissues which leads to the generation of a dysbiotic, subgingival biofilm community, and progression of periodontitis. The type 9 secretion system, lipid A modification, and the formation of outer membrane vesicles are important ...
Mike A. Curtis, James A. Garnett, Richard P. Darveau   +2 more
wiley   +1 more source

Constraints on lateral gene transfer in promoting fimbrial usher protein diversity and function [PDF]

Open Biology, 2017
Fimbriae are long, adhesive structures widespread throughout members of the family Enterobacteriaceae. They are multimeric extrusions, which are moved out of the bacterial cell through an integral outer membrane protein called usher.
Christopher J. Stubenrauch, Gordon Dougan, Trevor Lithgow, Eva Heinz   +3 more
doaj   +1 more source

Random Mutagenesis in Porphyromonas gingivalis Enables Isolation of Mutants With Enhanced Secreted Protease Activity

Molecular Oral Microbiology, EarlyView.
ABSTRACT Porphyromonas gingivalis is a key pathogen in periodontitis, with secreted proteases as major virulence factors. We developed a screening method to generate and identify P. gingivalis mutants with elevated protease activity. Mutations were induced using the mutagens 2,6‐diaminopurine (2,6‐DAP) or ethyl methanesulfonate (EMS), and the ...
Takeru Nakabayashi, Satoshi Yuhara, Kosei Tanaka   +2 more
wiley   +1 more source

Protection Against Pneumonia Induced by Vaccination with Fimbriae Subunits from Klebsiella pneumoniae

Vaccines
Background: Klebsiella pneumoniae infections pose a great burden worldwide, causing high morbidity and mortality, which are worsened by the increase in multidrug-resistant strains.
Lucas Assoni, Isabelle Ciaparin, Monalisa Martins Trentini, Juliana Baboghlian, Gabriel Rodrigo, Brenda Vieira Ferreira, José Aires Pereira, Carlos Martinez, Lucio Ferraz, Raquel Girardello, Lucas Miguel Carvalho, Anders P. Hakansson, Thiago Rojas Converso, Michelle Darrieux   +13 more
doaj   +1 more source

Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins

Bio-Protocol, 2013
This simple protocol describes how to detect antigens from agar-grown bacterial colonies transferred to nitrocellulose using specific antibodies. The protocol is well suitable for detection of bacterial proteins exposed on the cell surface or secreted to
Timo Lehti, Benita Westerlund-Wikström
doaj   +1 more source