Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Analysis of success factors in crowdfunding projects based on rewards: A way to obtain financing for socially committed projects [PDF]
, 2020 Cinta Borrero-Domínguez +2 more
openalex +1 more source
Fitting Health Financing Reforms to Context: Examining the Evolution of Results-Based Financing Models and the Slow National Scale-Up in Uganda (2003-2015) [PDF]
, 2021 Aloysius Ssennyonjo +3 more
openalex +1 more source
Salaries of Members of Congress: A List of Payable Rates and Effective Dates, 1789-2008 [PDF]
, 2008 Congress is required by Article I, Section 6, of the Constitution to determine its own pay. Prior to 1969, Congress did so by enacting stand-alone legislation. From 1789 through 1968, Congress raised its pay 22 times using this procedure.
Brudnick, Ida A. +1 more
core +2 more sources
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
2017 Trade Finance Gaps, Growth and Jobs Survey [PDF]
, 2017 Key Points • The global trade finance gap is estimated at $1.5 trillion. • 40% of the gap originates in Asia and the Pacific. • 74% of rejected trade finance transactions come from SMEs and midcap firms. • Female-owned firms report higher rejection rates,
Beck, Steven +2 more
core +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Why is finance critical? A dialogue with a women's community in Sri Lanka [PDF]
, 2009 The busrt of the bubble has given momentum to the search of escape routes from the current transnational financial system and its underlying principles.
Chandrasekara, I
core
FEBS Open Bio, EarlyView.Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar +3 more
wiley +1 more source
Journal of Financial and Quantitative Analysis, 2014 AbstractWe find that 43% of firms that make payouts also raise capital during the same year, resulting in 31% of aggregate payouts being externally financed, primarily with debt. Most financed payouts cannot be explained by payout smoothing in response to volatile earnings or investment (rather, they are the result of firms persistently setting payouts
Joan Farre-Mensa +2 more
openaire +2 more sources