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Synthetic firefly luciferins for bioluminescence imaging (要旨)

Synthetic firefly luciferins for bioluminescence imaging (要旨)
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Novel Heterocyclic Analogues of Firefly Luciferin

Biochemistry, 2012
Five novel firefly luciferin analogues in which the benzothiazole ring system of the natural substrate was replaced with benzimidazole, benzofuran, benzothiophene, benzoxazole, and indole were synthesized. The fluorescence, bioluminescence, and kinetic properties of the compounds were evaluated with recombinant Photinus pyralis wild type luciferase ...
Carolyn C, Woodroofe, Poncho L, Meisenheimer, Dieter H, Klaubert, Yumi, Kovic, Justin C, Rosenberg, Curran E, Behney, Tara L, Southworth, Bruce R, Branchini   +7 more
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Theoretical Study on Storage and Release of Firefly Luciferin

Photochemistry and Photobiology, 2021
AbstractAmong numerous bioluminescent organisms, firefly is the most studied one. Recent experiment proposed that sulfoluciferin (SLH2) may serve as a storage form of luciferin (LH2). In the present article, we employed density functional theory calculation to uncover the mechanism and detailed process of the storage and release reactions.
Ya‐Jie Guo, Cheng‐Xing Cui, Ya‐Jun Liu   +2 more
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Chemiluminescence of Firefly Luciferin without Enzyme

Science, 1962
We have been able to produce chemiluminescence in firefly luciferin without utilizing the enzyme luciferase. Following the analogous mechanism of the chemiluminescence of luminol in the organic solvent dimethyl sulfoxide, we have prepared synthetically the methyl ester of luciferin, the phosphate ester of luciferin and luciferyl adenylate ...
H H, SELIGER, W D, McELROY
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Differential Effect of Azetidine Substitution in Firefly Luciferin Analogues

ChemBioChem, 2021
AbstractReplacing an N,N‐dimethylamino group in a classical fluorophore with a four membered azetidine ring provides an improved luminescence quantum yield. Herein, we extended this strategy to bioluminescent firefly luciferin analogues and evaluated its general validity.
Yuma, Ikeda, Mariko, Orioka, Takahiro, Nomoto, Yuki, Hiruta, Nobuhiro, Nishiyama, Daniel, Citterio   +5 more
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Multicolor Bioluminescence Obtained Using Firefly Luciferin

Current Topics in Medicinal Chemistry, 2016
Firefly bioluminescence is widely used in life science research as a useful analysis tool. For example, the adenosine-5`-triphosphate (ATP)-dependent enzymatic firefly bioluminescence reaction has long been utilized as a microbial monitoring tool. Rapid and sensitive firefly luciferin-luciferase combinations are used not only to measure cell viability ...
Masahiro, Kiyama, Ryohei, Saito, Satoshi, Iwano, Rika, Obata, Haruki, Niwa, Shojiro A, Maki   +5 more
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Firefly luciferin as a multifunctional chemiluminescence molecule

Photochemical & Photobiological Sciences, 2013
The chemiluminescence properties of firefly luciferin were studied, in a variety of chemical systems different from those in which it is usually studied. It was found that luciferin is a multifunctional chemiluminescence molecule, as it can behave as a chemiluminescence sensitizer, an emitter and a substrate.
Luís, Pinto da Silva, Joaquim C G, Esteves da Silva   +1 more
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The preparation and properties of crystalline firefly luciferin

Archives of Biochemistry and Biophysics, 1957
Abstract 1. 1. The purification and preparation of crystalline firefly luciferin is described. From 15,000 firefly lanterns approximately 9 mg. of crystalline luciferin can be prepared. 2. 2. The empirical formula appears to be C13H12N2S2O3. Infrared spectra and microorganic spot tests of luciferin and various derivatives indicate that two of
B, BITLER, W D, McELROY
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A model for firefly luciferin biosynthesis

Journal of the Chemical Society, Chemical Communications, 1975
The suggestion that firefly luciferin arises from p-benzoquinone and cysteine in nature offers a synthesis of the benzothiazole nucleus which may be related to a possible biosynthesis.
Frank McCapra, Zia Razavi
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Dye induced quenching of firefly luciferase–luciferin bioluminescence

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2007
The quenching of firefly bioluminescence (BL) in presence of xanthene dyes and tetratolylporphyrin was investigated. The BL intensity was quenched with an altered decay pattern in presence of xanthene dyes and tetratolylporphyrin. The electronic absorption spectra indicate that there is no significant interaction occurring between the dyes and the BL ...
N V, KrishnaMurthy, T, Sudhaharan, A, Ram Reddy   +2 more
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