Results 71 to 80 of about 73,969 (236)

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Canonical Antibodies Adopt Distinct Binding Modes to Recognize Viral Glycan Shields

Advanced Science, EarlyView.
Canonical Y‐shaped antibodies recognize viral glycan shields through adaptive Fab assembly states shaped by glycan organization and somatic hypermutation. Structural analyses ofbroadly neutralizing antibodies VRC35 and VRC36 across glycoproteins of HIV‐1, influenza, SARS‐CoV‐2, and Lassa viruses reveal distinct Fab assembly states, spanning monovalent,
Jiaxuan Cheng, Evan M. Cale, Amirabbas Maghsoudi, Matthew S. Sutton, Nancy S. Longo, Rebecca A. Gillespie, Lingshu Wang, Ivan Kosik, Sue Chong, Atsuhiro Yasuhara, Tatsiana Bylund, Arne Schön, Prabhanshu Tripathi, Yaroslav Tsybovsky, Haotian Lei, Nicholas C. Morano, Allison B. Lupatkin, Abraham J. Morton, Zabrina C. Lang, Jordan E. Becker, Isabella R. Frascilla, Myungjin Lee, Ning Li, Cuiping Liu, Ryan S. Roark, Chen‐Hsiang Shen, I‐Ting Teng, David J. Van Wazer, Danyi Wang, Lingyuan Wu, Goran Ahlsen, Mike Castro, Haijuan Du, Michael J. Ernandes, Tiansheng Li, Bob C. Lin, Mark K. Louder, Krisha McKee, Jonah S. Merriam, Sijy O'Dell, Li Ou, Sergei Pletnev, David Prikryl, Qi Qiu, Sarah Rubin, Mallika Sastry, Stephen D. Schmidt, Asif Shajahan, Andrea R. Shiakolas, Sanjay Srivatsan, Baoshan Zhang, Qiong Zhou, Mark Connors, Jason G. Gall, Yicheng Guo, Rick K. Huang, Yaoxing Huang, Richard A. Koup, Q. Paula Lei, John R. Mascola, Reda Rawi, Leonid Serebryannyy, Lawrence Shapiro, Zizhang Sheng, David D. Ho, Patrick C. Wilson, Jonathan W. Yewdell, Theodore C. Pierson, Masaru Kanekiyo, Nicole A. Doria‐Rose, Peter D. Kwong, Tongqing Zhou   +71 more
wiley   +1 more source

Dual‐Organelle Secretome Conjugation and Organ‐Uptake Tracking (DuO‐SCOUT) Enables Deep and Sensitive Mapping of the Secreted Proteins

Advanced Science, EarlyView.
A novel dual‐organelle proximity labeling platform, DuO‐SCOUT, decodes the complex landscape of systemic organ‐organ communication by capturing both classical and unconventional secretomes. Application in metabolic models maps the adipose‐to‐brain secretory relay, identifying selective extra‐hypothalamic sites for adipose‐derived factors and expanding ...
Fenglian Yang, Rui Qian, Jiaxing Song, Xingqi Meng, Jin Young Kim, Kwok On Lai, Li Wang, Liang Zhang   +7 more
wiley   +1 more source

Tuning Local Modulus at Liquid Metal‐Polymer Interfaces Regulates Electrocaloric Effect in Ferroelectric Nanocomposites

Advanced Electronic Materials, EarlyView.
Liquid metal nanoparticles create mechanically compliant polymer interfaces that facilitate reversible dipolar reorientation under electric fields, whereas rigid interfaces impose stronger local constraints. This soft–rigid interface comparison reveals local interfacial modulus as a key parameter for tuning electrocaloric entropy change in ...
Tian Yao, Shanyu Zheng, Binzhou Sun, Zhenhua Ma, Feiyu Zhang, Lu Yu, Qiang Li, Ruhong Luo, Feihong Du, Yifan Zhao, Yezhan Lin, Chunyu Wang, Xin Chen, Shi‐Yang Tang, Tiannan Yang, Xiaoshi Qian   +15 more
wiley   +1 more source

Guidelines for High‐Throughput Screening for Natural Products With Anti‐Aging Activity Based on Drosophila Intestinal Homeostasis

AgriFood: Journal of Agricultural Products for Food, EarlyView.
ABSTRACT Aging represents a critical risk factor for chronic diseases, driving an urgent demand for interventions promoting healthy longevity. This study establishes standardized guidelines for high‐throughput screening (HTS) of anti‐aging natural products using Drosophila melanogaster, leveraging its intestinal homeostasis as a biomarker.
Xiao Sheng, Lei Zhang
wiley   +1 more source

Analysis of piscicultural-biological results of works with Russian sturgeon brood fish at the sturgeon hatchery “Lebyazhy” (Astrakhan region, Russia)

Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ, 2013
The state of world stocks of sturgeons is on the edge of catastrophe. These species are either extinct or under threat of extinction under human impacts.
R. V. Kononenko
doaj   +1 more source

FISH EGGS AND LARVAE fROM THE JAVA-SEA

Treubia, 2014
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openaire   +1 more source

Staging an Experience of Cultural Heritage Preservation: Consumers' Willingness to Pay for Heirloom Rice in the Philippines

Agribusiness, EarlyView.
ABSTRACT The Cordillera Administrative Region in the Philippines is home to terraced rice embedded in centuries of cultural heritage. However, weak market incentives threaten sustained production, jeopardizing indigenous communities' cultural heritage and the in situ biodiversity of rice genetic resources.
Kofi Britwum, Matty Demont
wiley   +1 more source

Studies on the fecundity of Mastacembelus armatus fish from River Narmada in West Nimar (M.P.)

Environment Conservation Journal, 2014
The spiny eel Mastacembelus armatus is an economically important freshwater food fish native of India, Pakistan, Sri Lanka, Thailand, Indonesia and other parts of South-east Asia.
Sunita Bakawale, R. R. Kanhere
doaj   +1 more source

Quantifying dominant bacterial genera detected in metagenomic data from fish eggs and larvae using genus-specific primers. [PDF]

Microbiologyopen, 2022
Najafpour B, Pinto PIS, Canario AVM, Power DM.   +3 more
europepmc   +1 more source