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‘Messenger RNA’ in a Fission Yeast
Nature, 1962IT is now widely believed that one fraction of the ribonucleic acid (RNA) of a cell, the ‘messenger RNA’, is responsible for the transmission of genetic information from the deoxyribonucleic acid (DNA) of the nucleus to the cytoplasm1. This messenger RNA has been identified with that fraction of the total RNA which shows some or all of the following ...
P. R. Gross+2 more
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Cell, 1988
Genetic studies of the fission yeast cell cycle have identified the cdc2 gene as a critical regulator of M phase (Nurse and Thuriaux, 1980). The gene encodes a protein kinase (Simanis and Nurse, 1986) that is homologous to the product of the CDC28 cell cycle "start" gene of budding yeast (Beach et al., 1982).
Robert Booher+3 more
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Genetic studies of the fission yeast cell cycle have identified the cdc2 gene as a critical regulator of M phase (Nurse and Thuriaux, 1980). The gene encodes a protein kinase (Simanis and Nurse, 1986) that is homologous to the product of the CDC28 cell cycle "start" gene of budding yeast (Beach et al., 1982).
Robert Booher+3 more
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Sexual differentiation in fission yeast
Trends in Genetics, 1990The regulation of sexual reproduction in yeast constitutes the highest level of differentiation observed in these unicellular organisms. The various ramifications of this system involve DNA rearrangement, transcriptional control, post-translational modification (such as protein phosphorylation) and receptor/signal processing.
Dietmar Weilguny+2 more
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Electron Microscopy of Fission Yeast
Cold Spring Harbor Protocols, 2017Electron microscopy (EM) can provide images of cells with a spatial resolution that significantly surpasses that available from light microscopy (LM), even with modern methods that give LM “super resolution.” However, EM resolution comes with costs in time spent with sample preparation, expense of instrumentation, and concerns regarding sample ...
Mary K. Morphew+2 more
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TOR Signaling in Fission Yeast
Critical Reviews in Biochemistry and Molecular Biology, 2008Fission yeast has two TOR kinases, Tor1 and Tor2. Recent studies have indicated that this microbe has a TSC/Rheb/TOR pathway like higher eukaryotes. Two TOR complexes, namely TORC1 and TORC2, have been identified in this yeast, as in budding yeast and mammals.
Yo ko Otsubo, Masayuki Yamamato
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Tetrad Dissection in Fission Yeast
2018Tetrad dissection is a powerful tool in yeast genetics that allows the analysis of products of a single meiosis. With just a few tetrads, it is possible to determine linkage, identify unique phenotypes associated with double mutants, or assess specific meiotic defects. Strains are crossed on nitrogen-limiting medium for 3 days.
Susan L. Forsburg, Wilber Escorcia
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Oxygen toxicity in a fission yeast
Journal of Cellular Physiology, 1971AbstractContinuous exposure of synchronous cultures of Schizosaccharomyces pombe to 2.0 atmospheres oxygen beginning at any point in the first two‐thirds of the cell cycle prevented subsequent cell division. Similar exposure during the last one‐third of the cell cycle did not prevent cell division.
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Centromeres of budding and fission yeasts
Trends in Genetics, 1990Centromeres of the budding yeast Saccharomyces cerevisiae are structurally relatively simple, are specified by only about 125 base pairs of DNA, and contain no repeated DNA sequences. The centromere regions in the fission yeast Schizosaccharomyces pombe span many kilobase pairs of DNA and contain repeated DNA sequences that appear to be necessary for ...
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Chromatin Immunoprecipitation in Fission Yeast
2011A tremendous amount of information regarding the nature and regulation of heterochromatin has emerged in the past 10 years. This rapid progress is largely due to the development of techniques such as chromatin immunoprecipitation or "ChIP," which allow analysis of chromatin structure. Further technological advances such as microarray analysis and, more
Tom Volpe, Jessica DeMaio
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Centromeric chromatin in fission yeast
Frontiers in Bioscience, 2008A fundamental requirement for life is the ability of cells to divide properly and to pass on to their daughters a full complement of genetic material. The centromere of the chromosome is essential for this process, as it provides the DNA sequences on which the kinetochore (the proteinaceous structure that links centromeric DNA to the spindle ...
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