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Transcriptional silencing in fission yeast
Journal of Cellular Physiology, 2000In eukaryotes, epigenetic events govern diverse processes, ranging from gene expression to other aspects of global chromosome architecture essential for preserving the integrity of the genome. Transcriptional silencing at the mating-type locus, centromeres, and telomeres of the fission yeast is regulated by epigenetic mechanisms.
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Ribosome Numbers in a Fission Yeast
Nature, 1965THERE is a need for a more confined and precise use of the word ribosome. It would clarify molecular biology if the word were reserved for particles in the cell which measure 100 A–300 A in diameter, are composed of RNA and protein, and have been shown to be active as sites for the synthesis of proteins.
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Centromeric chromatin in fission yeast
Frontiers in Bioscience, 2008A fundamental requirement for life is the ability of cells to divide properly and to pass on to their daughters a full complement of genetic material. The centromere of the chromosome is essential for this process, as it provides the DNA sequences on which the kinetochore (the proteinaceous structure that links centromeric DNA to the spindle ...
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TOR Signaling in Fission Yeast
Critical Reviews in Biochemistry and Molecular Biology, 2008Fission yeast has two TOR kinases, Tor1 and Tor2. Recent studies have indicated that this microbe has a TSC/Rheb/TOR pathway like higher eukaryotes. Two TOR complexes, namely TORC1 and TORC2, have been identified in this yeast, as in budding yeast and mammals.
Yoko, Otsubo, Masayuki, Yamamato
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Analysis of chromatin in fission yeast
Methods, 2004The use of fission yeast as a model system for studies of chromosome biology has contributed to several key advances in the last few years. The structure of its large complex centromeres and composition of its transcriptionally silent heterochromatin resemble those of metazoa.
Alison, Pidoux +2 more
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Electron Microscopy of Fission Yeast
Cold Spring Harbor Protocols, 2017Electron microscopy (EM) can provide images of cells with a spatial resolution that significantly surpasses that available from light microscopy (LM), even with modern methods that give LM “super resolution.” However, EM resolution comes with costs in time spent with sample preparation, expense of instrumentation, and concerns regarding sample ...
J Richard, McIntosh +2 more
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Oxygen toxicity in a fission yeast
Journal of Cellular Physiology, 1971AbstractContinuous exposure of synchronous cultures of Schizosaccharomyces pombe to 2.0 atmospheres oxygen beginning at any point in the first two‐thirds of the cell cycle prevented subsequent cell division. Similar exposure during the last one‐third of the cell cycle did not prevent cell division.
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Centromeres of budding and fission yeasts
Trends in Genetics, 1990Centromeres of the budding yeast Saccharomyces cerevisiae are structurally relatively simple, are specified by only about 125 base pairs of DNA, and contain no repeated DNA sequences. The centromere regions in the fission yeast Schizosaccharomyces pombe span many kilobase pairs of DNA and contain repeated DNA sequences that appear to be necessary for ...
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Tetrad Dissection in Fission Yeast
2018Tetrad dissection is a powerful tool in yeast genetics that allows the analysis of products of a single meiosis. With just a few tetrads, it is possible to determine linkage, identify unique phenotypes associated with double mutants, or assess specific meiotic defects. Strains are crossed on nitrogen-limiting medium for 3 days.
Wilber, Escorcia, Susan L, Forsburg
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Fission yeast on the brink of meiosis
BioEssays, 2000The fission yeast Schizosaccharomyces pombe (S. pombe) is now well established as a versatile genetic model organism. It is widely used to analyse the basic eukaryotic cell cycle during vegetative growth and it is also well suited to studies on the elementary processes of sexual reproduction, including intercellular communication and signal ...
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