Results 141 to 150 of about 114,985 (325)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Classes of ODE solutions: smoothness, covering numbers, implications for noisy function fitting, and the curse of smoothness phenomenon [PDF]
, 2020 Ying Zhu, Mozhgan Mirzaei
openalex +1 more source
Fitting Classes and Formations with Desired Properties of Hall Subgroups [PDF]
, 2015 В данной работе конструируются новые семейства классов конечных π-разрешимых групп: классы Фиттинга, опреде-ляемые вложением холловых π-подгрупп в радикалы групп, и формации, определяемые вложением корадикалов групп в хол-ловы π-подгруппы и холловых π ...
Воробьев, Н. Т. +1 more
core
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
ClusterVAR: Fitting Latent Class Vector-Autoregressive (VAR) Models [PDF]
Anja F. Ernst, Jonas M B Haslbeck
openalex +1 more source
The Astrophysical JournalPolycyclic aromatic hydrocarbons (PAHs) are fundamental to understanding the interstellar medium of several astrophysical objects. Normally present in starburst (SB) galaxies, they have also been more frequently detected in active galaxy nuclei (AGNs ...
Carla M. Canelo +5 more
doaj +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source