Results 121 to 130 of about 5,846,948 (298)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Fixed point theorems for set-valued contraction type maps in metric spaces
, 2010 We first give some fixed point results for set-valued self-map contractions in complete metric spaces. Then we derive a fixed point theorem for nonself set-valued contractions which are metrically inward. Our results generalize many well-known results in
Amini-Harandi, A, O'Regan, D
core +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Fixed Point Results in Convex Double-Controlled Metric-Type Spaces and Applications
MathematicsThis paper investigates fixed point results in convex double-controlled metric-type spaces. By introducing a convex structure on double-controlled metric-type spaces, we study the convergence of the Mann iteration process for contractive mappings in this
Nazli Kadioglu Karaca
doaj +1 more source
Existence and Uniqueness of a Fixed Point for the Bellman Operator in Deterministic Dynamic Programming [PDF]
We study existence and uniqueness of a fixed point for the Bellman operator in deterministic dynamic programming. We show that removing many of the assumptions of the theorem on the Bellman operator recently shown by Martins-da-Rocha and Vailakis ...
Takashi Kamihigashi
core
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Measures of weak noncompactness and fixed point theory in banach algebras satisfying condition (P)
, 2017 The aim of this paper is to prove some new fixed point theorems in a nonempty closed convex subset of a Banach algebra satisfying a sequential condition (P) in a weak topology setting.peer ...
O’Regan, Donal, Ben Amar, Afif
core +1 more source