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Bacterial flagellin—a potent immunomodulatory agent [PDF]

open access: yesExperimental and Molecular Medicine, 2017
Flagellin is a subunit protein of the flagellum, a whip-like appendage that enables bacterial motility. Traditionally, flagellin was viewed as a virulence factor that contributes to the adhesion and invasion of host cells, but now it has emerged as a potent immune activator, shaping both the innate and adaptive arms of immunity during microbial ...
Irshad A Hajam   +2 more
exaly   +3 more sources
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Sequential polymerization of flagellin A and flagellin B into Caulobacter flagella

Journal of Molecular Biology, 1981
The mode of polymerization of two species of flagellins, flagellin A and flagellin B, in polar flagella of Caulobacter crescentus was examined. By immunological staining we found that 1 to 1.2 μm of the portion of the flagellar filament proximal to the cell was composed of flagellin B, whereas about 5 μm of the distal portion was composed of flagellin ...
S, Koyasu, M, Asada, A, Fukuda, Y, Okada
openaire   +2 more sources

Purification of Native Flagellin

2019
Flagella are effective organelles of locomotion and one of several virulence factors in Proteus mirabilis. To study their properties and role in virulence, we describe a protocol to extract and purify the native flagellin of P. mirabilis. Purified flagellin can be visualized by SDS-PAGE or immunoblot and is suitable for downstream applications such as ...
María José, González   +3 more
openaire   +2 more sources

Flagellin Signalling in Plant Immunity

2007
Like all higher living organisms, plants are constantly exposed to microbes that either grow epiphytically on the organ surface, establish beneficial interactions in specific tissues, or infect host tissues as pathogens and cause disease. In order to infect, pathogens must attach to the plant surface and break physical barriers to enter the tissue ...
Chinchilla, D., Boller, T., Robatzek, S.
openaire   +3 more sources

Flagellin maintains eosinophils in the intestine

Cytokine, 2022
Eosinophils (Eos) are the major effector cells in allergic response. The regulation of Eo is not fully understood yet. Flagellin (FGN) has immune regulatory functions. This study aims to elucidate the role of FGN in maintaining Eo at the static status in the intestinal tissues. A mouse food allergy (FA) model was developed.
Xiaodan, Lv   +7 more
openaire   +2 more sources

Flagellin Takes Its Toll

Science Signaling, 2012
Bacterially derived flagellin binds to an innate immune receptor to form a tail-to-tail heterodimeric signaling complex.
openaire   +2 more sources

Flagellin Binding by FLS2

Science's STKE, 2001
Immune responses to flagellin have been evolutionarily conserved between plants and animals. In Arabidopsis , FLS2, a leucine-rich repeat (LRR) domain containing serine-threonine transmembrane kinase binds bacterial flagellin and mediates signals, culminating in a defensive response. Gómez-Gómez et al.
openaire   +2 more sources

TLR5 Tolls for Flagellin

Science Signaling, 2001
Hayashi et al. characterized the transmembrane Toll-like receptor (TLR) 5 protein and identified a physiologically relevant ligand capable of activating TLR5 signaling. Overexpression of chimeric proteins consisting of the intracellular domain of TLR5 fused to the extracellular domain of CD4 (to promote dimerization)
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Relatedness of the flagellins from methanogens

Archives of Microbiology, 1992
Purified flagellar filaments isolated from six methanogens were composed of multiple flagellins. Two flagellins were present in Methanococcus deltae (Mr = 34,000 and 32,000), Methanoculleus marisnigri (Mr = 31,000 and 25,500) and Methanococcus jannaschii (Mr = 31,000 and 27,500), three in Methanothermus fervidus (Mr = 34,000, 25,000 and 24,000) and ...
M L, Kalmokoff, S F, Koval, K F, Jarrell
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Characterization of flagellin from Clostridium chauvoei

Veterinary Microbiology, 1999
Differential centrifugation and cesium chloride-equilibrium centrifugation were used to purify the flagella from the strain Okinawa of the formalin-fixed Clostridium chauvoei. SDS-PAGE profile of purified flagella showed that a major protein band with a molecular mass of 46 kDa, corresponding to the flagellin monomer, and at least two minor protein ...
A, Kojima   +3 more
openaire   +2 more sources

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