Results 31 to 40 of about 5,671 (202)

Germinal proto-mitochondria from rat liver

open access: yesBiochemistry and Biophysics Reports, 2019
A number of properties of the smallest (less than 0.2 μm) germinal proto-mitochondria (PRMC) from rat liver have been studied. These PRMC were obtained by filtering the light fraction of hepatic mitochondria (MC) through calibrated millipore membranes ...
Nikolai Vekshin   +2 more
doaj   +1 more source

Activation of a Flavoprotein by Proteolysis

open access: yesJournal of Biological Chemistry, 1989
Chymotryptic digestion of brain pyridoxine-5-P oxidase brings about a 4-fold enhancement of the catalytic power (Vmax/KM) using pyridoxine-5-P as substrate in the assay mixtures. The chymotrypsin-treated enzyme is less susceptible to inhibition by pyridoxal-5-P than the native enzyme.
Y T, Kim, J E, Churchich
openaire   +2 more sources

Structures and mechanism of the monoamine oxidase family

open access: yesBiomolecular Concepts, 2011
Members of the monoamine oxidase family of flavoproteins catalyze the oxidation of primary and secondary amines, polyamines, amino acids, and methylated lysine side chains in proteins.
Gaweska Helena, Fitzpatrick Paul F.
doaj   +1 more source

Investigation of real-time photorepair activity on DNA via surface plasmon resonance.

open access: yesPLoS ONE, 2012
The cyclobutane pyrimidine dimer (CPD) and 6-4 lesion formations along with the specific breaks on strands are the most common type of DNA damage caused by Ultraviolet light (UV) irradiation.
Rıza Kizilel   +5 more
doaj   +1 more source

Engineering an Artificial Flavoprotein Magnetosensor [PDF]

open access: yesJournal of the American Chemical Society, 2016
Migratory birds use the Earth's magnetic field as a source of navigational information. This light-dependent magnetic compass is thought to be mediated by cryptochrome proteins in the retina. Upon light activation, electron transfer between the flavin adenine dinucleotide cofactor and tryptophan residues leads to the formation of a spin-correlated ...
Chris Bialas   +9 more
openaire   +2 more sources

Adaptive Laboratory Evolution of Flavin Functionality Identifies Dihydrolipoyl Dehydrogenase as One of the Critical Points for the Activity of 7,8-Didemethyl-Riboflavin as a Surrogate for Riboflavin in Escherichia coli

open access: yesMolecules
Riboflavin analogs lacking one methyl group (7α or 8α) can still serve as a surrogate for riboflavin in riboflavin-deficient microorganisms or animals. The absence of both methyl groups at once completely abolishes this substitution capability.
Farshad La-Rostami   +8 more
doaj   +1 more source

Two-photon microscopy for non-invasive, quantitative monitoring of stem cell differentiation. [PDF]

open access: yesPLoS ONE, 2010
The engineering of functional tissues is a complex multi-stage process, the success of which depends on the careful control of culture conditions and ultimately tissue maturation.
William L Rice   +2 more
doaj   +1 more source

Mechanisms of flavoprotein‐catalyzed reactions [PDF]

open access: yesEuropean Journal of Biochemistry, 1989
Flavoproteins are a class of enzymes catalyzing a very broad spectrum of redox processes by different chemical mechanisms. This review describes the best studied of these mechanisms and discusses factors possibly governing reactivity and specificity.
Ghisla, Sandro, Massey, Vincent
openaire   +5 more sources

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

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