Results 131 to 140 of about 834,813 (315)

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

open access: yesFEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

open access: yesFEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson   +6 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Mass Flow Rate Characteristic of the Flapper-nozzle Pneumatic Valve [PDF]

open access: yes, 2015
Flapper-nozzle type valve is commonly used for precision, flow control in pneumatic systems. For the purpose of analysis and design the paper is concerned with nonlinear mass flow rate of the valve taking into account different flow regimes. Flow rate is
Dubonjic, Ljubisa   +2 more
core  

A Novel Flow Characteristic Regulation Method for Two-Stage Proportional Valves Based on Variable-Gain Feedback Grooves

open access: yesMachines
The two-stage proportional valve is a key control component in heavy-duty equipment, where its signal-flow characteristics critically influence operational performance.
Xingyu Zhao   +5 more
doaj   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

open access: yesFEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry   +9 more
wiley   +1 more source

Flow and combustion in disc and pent-roof SI engines [PDF]

open access: yes, 2006
Reported in this thesis is a study of combustion in a disc-shaped combustion chamber spark ignition engine, and in-cylinder flow and combustion in an idealised pent-roof spark ignition engine. Both engines were skip fired, to remove residuals and ensure
Murad, Ahmad E M A, Murad, Ahmad E. M. A
core  

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

open access: yesFEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Analysis and Experimental Study of the Flow Characteristics in a Micro-Channel [PDF]

open access: yesInternational Journal Bioautomation, 2014
To study the flow characteristics of low-speed fluid in a micro-channel, a model including the silk gland middle section, transition section, and forepart of a silkworm silk gland was established.
Xiaorong Wang   +4 more
doaj  

Design and analysis strategies for robust microbiome ageing research

open access: yesFEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik   +5 more
wiley   +1 more source

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