Results 131 to 140 of about 799,277 (314)

Rankl genetic deficiency and functional blockade undermine skeletal stem and progenitor cell differentiation

Stem Cell Research & Therapy
Background Skeletal Stem Cells (SSCs) are required for skeletal development, homeostasis, and repair. The perspective of their wide application in regenerative medicine approaches has supported research in this field, even though so far results in the ...
M. L. Schiavone, L. Crisafulli, C. Camisaschi, G. De Simone, F. R. Liberati, E. Palagano, N. Rucci, F. Ficara, Cristina Sobacchi   +8 more
doaj   +1 more source

Flow Cytometric Enumeration of the Blood Cells of Rainbow Trout (Oncorhynchus mykiss) and New Zealand Freshwater Crayfish (Paranephrops planifrons) [PDF]

, 2009
The aim of this study was to develop flow cytometric (FC) methods to enumerate rainbow trout (Oncorhynchus mykiss) whole blood cells and New Zealand freshwater crayfish (Paranephrops planifrons) haemocytes as non-lethal endpoints in the evaluation of ...
Taylor, Sean Charles
core  

Gellan Gum Fluid Gel System for Controlled‐Delivery of Cytokine‐Licensed MSC‐EVs to Enhance Corneal Repair in Limbal Stem Cell Deficiency

Advanced Healthcare Materials, EarlyView.
Gellan gum fluid gel (FG) enables controlled ocular delivery of mesenchymal stromal cell (MSC)‐derived extracellular vesicles (EV). Its shear‐thinning, solid‐liquid‐solid rheology ensures prolonged ocular retention. Sustained MSC‐EV release (∼18% in 6 hrs) supports effective EV ocular delivery.
Seyedmohammad Moosavizadeh, Ava Dashti, Thomas E. Robinson, Robyn Gaffey, Richard Moakes, Neil Lagali, Liam Grover, Thomas Ritter   +7 more
wiley   +1 more source

Therapeutic Extracellular Vesicles from Synovial Fibroblast‐Primed MSCs for Osteoarthritis Treatment

Advanced Healthcare Materials, EarlyView.
EVs derived from the microenvironment of SFs transferred MSCs (miSF‐MSC‐EVs) enhanced the expression of miRNAs that regulate cell migration, cell adhesion, and the TGF‐β signaling pathway, demonstrating superior anti‐inflammatory and cartilage regeneration effects compared to conventional MSC‐EVs.
Seo Jeong Kim, Ji Seob Kim, Min Hee Moon, Jae‐Hyeok Jang, In Sun Hwang, Byung‐Hyun Cha   +5 more
wiley   +1 more source

Evaluating the utility of a nanoscale flow cytometer for detection of surface proteins on HIV and extracellular vesicles

Virology Journal
Background Flow virometry (FV) – the application of flow cytometry to viruses – has historically been hindered by the inability of cytometers to detect particles below ~ 300 nm in size.
Jonathan Burnie, Caroline Ouano, Vanessa Costa, Irene Castrosin, Catherine Hammond, Hannah Matthews, John Tigges, Kizzmekia S. Corbett-Helaire   +7 more
doaj   +1 more source

Gardiquimod Nanoemulsion Targets Cutaneous Leishmaniasis Lesions Reducing Systemic Toxicity and Parasite Burden

Advanced Healthcare Materials, EarlyView.
Nanoemulsion delivery of a TLR7 agonist enhances safety and promotes colocalization with Leishmania‐infected macrophages in skin lesions, enabling a ∼2‐log reduction in parasite burden. However, tissue‐level constraints and TLR‐driven regulatory feedback generate a mixed immune response that limits complete parasite clearance.
Carmen Palomino‐Cano, M. Carmen Mera‐Delgado, Esther Moreno, Esther Larrea, Lecnia Aguirre, Jonathan Miguel Zanatta, Sónia Barros‐Carvalho, Iola F. Duarte, Ricardo Silvestre, Juan M. Irache, Javier Carrión, Socorro Espuelas   +11 more
wiley   +1 more source

A new method for detecting mycoplasma in cell cultures using colocalization of DNA and membrane staining

Biochemistry and Biophysics Reports
Mycoplasma often surreptitiously contaminates cell cultures without detection, mainly parasitizing the cell surface and thus interfering to some extent with the cells.
Mengyuan Li, Shan Liu, Cheng Zhou, Mengsi Sun, Yanjie Wang   +4 more
doaj   +1 more source

Nanoplatforms for Sentinel and Tumor‐Draining Lymph Node Mapping: From Visualization to Pathway‐Informed Staging

Advanced Healthcare Materials, EarlyView.
Lymphotropic nanoplatforms integrate multimodal imaging with therapeutic guidance to map sentinel and tumor‐draining lymph nodes, revealing functional drainage, metastatic involvement, and nodal accessibility, while providing mechanistic insight to support precision surgical and therapeutic interventions.
Xu Chen, Nina Li, Meiyan Zou, Zihao Zhou, Rongwei Xu, Weiyao Feng, Xinyuan Zhao, Li Cui   +7 more
wiley   +1 more source

CD25+CD27+CD70− alloantigen-specific Tregs: promising stable immunotherapy for transplantation

Frontiers in Immunology
The clinical translation of alloantigen-specific regulatory T cells (AS-Tregs) is constrained by their low frequency in peripheral blood, limited purity, and functional instability following prolonged ex vivo expansion.
Arimelek Cortés-Hernández, Arimelek Cortés-Hernández, Saúl Arteaga-Cruz, Saúl Arteaga-Cruz, Iyari I. Martínez Iturbe, Iyari I. Martínez Iturbe, Katya Rosas-Cortina, Marco A. Vigil Mora, Marco A. Vigil Mora, Erick Legorreta-Anguiano, Erick Legorreta-Anguiano, Judith E. Reyes Barrientos, Judith E. Reyes Barrientos, Evelyn K. Álvarez-Salazar, Evelyn K. Álvarez-Salazar, Alejandra Cervera, Beatriz E. Sánchez-Hernández, Armando Gamboa Domínguez, Gloria Soldevila, Gloria Soldevila   +19 more
doaj   +1 more source

Temporal Immune and Metabolic Shifts Drive the Anti‐Tumor Efficacy of Resiquimod‐Loaded Nanoparticles in Peritoneal Carcinomatosis

Advanced Healthcare Materials, EarlyView.
Peritoneal carcinomatosis is a hard‐to‐treat spread of abdominal cancers that often returns after therapy. Nanoparticles were developed to retain an immune‐boosting drug in the abdomen. When combined with chemotherapy in mice, this approach eliminated tumors in many cases and prevented recurrence, helping the immune system mount a stronger, longer ...
Vanessa Chan, Po‐Han Chao, XuXin Sun, Jun Han, Sarah MacPherson, Lucas J. Andrew, Noah Y. Brittain, Tien Do, Marcel B. Bally, Julian J. Lum, Shyh‐Dar Li   +10 more
wiley   +1 more source