Results 121 to 130 of about 4,601,994 (298)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Identifying source of predictability for vapor pressure deficit variability in the southwestern United States

open access: yesnpj Climate and Atmospheric Science
Atmospheric vapor pressure deficit (VPD) measures the difference between saturation vapor pressure and actual vapor pressure, and its variability is closely related to fire activity in the western United States (US). Here, we assess the forecast skill of
Jiale Lou   +3 more
doaj   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

open access: yesFEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton   +6 more
wiley   +1 more source

Geophysical Fluid Dynamics Laboratory Gallery

open access: yes
Offered by the Geophysical Fluid Dynamics Laboratory (GFDL), the GFDL Gallery is a collection of visualizations which have been prepared in the course of GFDL's research, and subsequently presented at meetings and in the media.

core  

Parallel computational fluid dynamics : implementations and results using parallel computers : proceedings of the Parallel CFD '95 Conference, Pasadena, CA, U.S.A., 26-29 June, 1995 /

open access: yes, 1996
Parallel Computational Fluid Dynamics(CFD) is an internationally recognised fast-growing field. Since 1989, the number of participants attending Parallel CFD Conferences has doubled. In order to keep track of current global developments, the Parallel CFD
Parallel CFD Conference(1995 :Pasadena, Calif.)   +1 more
core  

Adenosine triphosphate as a modulator of protein interactions and stability

open access: yesFEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Consistency Analysis of Fluid-Structure Interaction Algorithms

open access: yes
LINComputational Fluid Dynamics ...
Blom, Frederic, Leyland, Pénélope
core  

The Land Component LM4.1 of the GFDL Earth System Model ESM4.1: Model Description and Characteristics of Land Surface Climate and Carbon Cycling in the Historical Simulation

open access: yesJournal of Advances in Modeling Earth Systems
We describe the baseline model configuration and simulation characteristics of the Geophysical Fluid Dynamics Laboratory (GFDL)'s Land Model version 4.1 (LM4.1), which builds on component and coupled model developments over 2013–2019 for the coupled ...
E. Shevliakova   +19 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

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