Results 61 to 70 of about 373,687 (257)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Motion planning for hyper-redundant manipulator systems: combining path following and improved APF-RRT* Path finding

Complex & Intelligent Systems
Hyper-redundant manipulator systems (HRMSs) have significant applications in various scenarios. Due to their excessive degrees of freedom (DOF), they can navigate into confined spaces and perform tasks such as exploration, detection, and maintenance ...
Hongcheng Ji, Cheng Wang, Chengzhen Wang, Haibo Xie   +3 more
doaj   +1 more source

Physical Extraction and Feature Fusion for Multi-Mode Signals in a Measurement System for Patients in Rehabilitation Exoskeleton

Sensors, 2018
Measurement system of exoskeleton robots can reflect the state of the patient. In this study, we combined an inertial measurement unit and a visual measurement unit to obtain a repeatable fusion measurement system to compensate for the deficiencies of ...
Canjun Yang, Qianxiao Wei, Xin Wu, Zhangyi Ma, Qiaoling Chen, Xin Wang, Hansong Wang, Wu Fan   +7 more
doaj   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Multi-Objective Optimization of VBHF in Deep Drawing Based on the Improved QO-Jaya Algorithm

Chinese Journal of Mechanical Engineering
Blank holder force (BHF) is a crucial parameter in deep drawing, having close relation with the forming quality of sheet metal. However, there are different BHFs maintaining the best forming effect in different stages of deep drawing.
Xiangyu Jiang, Zhaoxi Hong, Yixiong Feng, Jianrong Tan   +3 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Investigation of flow-induced noise reduction in high-specific-speed centrifugal pumps using bionic blades

Scientific Reports
To reduce the flow-induced noise of centrifugal pumps and reveal the noise source characteristics inside the pumps, this paper designs a bionic blade based on the “tubercle effect” of humpback whales.
Yongxin Jin, Tao Cheng, Jie Fu, Guangtai Shi, Lei He   +4 more
doaj   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Integrated MADM of low-carbon structural design for high-end equipment based on attribute reduction considering incomplete interval uncertainties

Scientific Reports
With the increasingly severe energy supply and environmental pressures, high-end equipment is gradually adopted to reduce the carbon emissions of manufacturing industry which makes its low-carbon structural design a critical research hotspot.
Zhaoxi Hong, Kaiyue Cui, Yixiong Feng, Jinyuan Song, Bingtao Hu, Jianrong Tan   +5 more
doaj   +1 more source