Results 91 to 100 of about 15,097 (295)
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
A Constrained Particle Dynamics for Continuum-Particle Hybrid Method in Micro- and Nano-Fluidics
, 2006 A hybrid method of continuum and particle dynamics is developed for micro- and nano-fluidics, where fluids are described by a molecular dynamics (MD) in one domain and by the Navier-Stokes (NS) equations in another domain.
崔佳 +3 more
core +1 more source
Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
FEBS Open Bio, EarlyView.Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Plug actuation and active manipulation in closed monolithic fluidics using backpressure [PDF]
, 2019 We explore the mechanisms to actuate and manipulate liquid plugs in monolithic closed channel fluidics with porous hydrophobic walls. Applying a small pressure, as much as 10 mbar, from the rear side of the porous wall, hereafter backpressure, the ...
G. Kokkoris +7 more
core +2 more sources
On-chip flow cytometer using integrated photonics for the detection of human leukocytes
Scientific ReportsDifferentiation between leukocyte subtypes like monocytes and lymphocytes is essential for cell therapy and research applications. To guarantee the cost-effective delivery of functional cells in cell therapies, billions of cells must be processed in a ...
Stijn Jooken +14 more
doaj +1 more source
Flow Cell Design for Effective Biosensing
Sensors, 2012 The efficiency of three different biosensor flow cells is reported. All three flow cells featured a central channel that expands in the vicinity of the sensing element to provide the same diameter active region, but the rate of channel expansion and ...
Douglas I. Stewart +3 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Charge Regulation at a Nanoporous Two-Dimensional Interface
ACS Omega, 2021 Mandakranta Ghosh +8 more
doaj +1 more source