Results 51 to 60 of about 432,570 (333)

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

Ocular surface staining: Current concepts and techniques

Indian Journal of Ophthalmology, 2023
The health of the ocular surface is vital for clear vision and comfort. Various factors can adversely influence the ocular surface and tear film homeostasis, and these include procedures like cataract and corneal refractive surgery.
Sanjeev P Srinivas, Srinivas K Rao
doaj   +1 more source

Conformational Dynamics of the Plug Domain of the SecYEG Protein-conducting Channel [PDF]

, 2011
The central pore of the SecYEG preprotein-conducting channel is closed at the periplasmic face of the membrane by a plug domain. To study its conformational dynamics, the plug was labeled site-specifically with an environment-sensitive fluorophore.
Arkowitz, Baneyx, Becker, Bol, Bonardi, Bondar, Cabelli, De Keyzer, Driessen, du Plessis, Duong, Egea, Evans, Fekkes, Frauenfeld, Greenough, Gumbart, Hanahan, Harris, Joly, Junne, Klose, Kusters, Lakowicz, Li, Lin, Lycklama, Maillard, Manting, Ménétret, Nakatogawa, Osborne, Plath, Prinz, Ramachandran, Rutkowska, Schaffitzel, Smith, Tam, Tsukazaki, Van den Berg, van der Does, van der Sluis, Zhang, Zimmer   +44 more
core   +2 more sources

Microporous Microgel Assemblies Facilitating the Recruitment and Osteogenic Differentiation of Progenitor Cells for Bone Regeneration

Advanced Functional Materials, EarlyView.
There is a significant need for biomaterials with well‐defined stability and bioactivity to support tissue regeneration. In this study, we developed a tunable microgel platform that enables the decoupling of stiffness from porosity, thereby promoting bone regeneration.
Silvia Pravato, Haishuo Guan, Bianca M. Carrara, Mira Jacobs, Matteo Monticelli, Lukas Moser, Thomas Moragues, Andrew J. deMello, Tomaso Zambelli, Martin Ehrbar   +9 more
wiley   +1 more source

Tracking of a Fluorescent Dye in a Freshwater Lake with an Unmanned Surface Vehicle and an Unmanned Aircraft System

Remote Sensing, 2018
Recent catastrophic events in our oceans, including the spill of toxic oil from the explosion of the Deepwater Horizon drilling rig and the rapid dispersion of radioactive particulates from the meltdown of the Fukushima Daiichi nuclear plant, underscore ...
Craig Powers, Regina Hanlon, David G. Schmale   +2 more
doaj   +1 more source

Quantitative photoluminescence of broad band absorbing melanins: A procedure to correct for inner filter and re-absorption effects

, 2004
We report methods for correcting the photoluminescence emission and excitation spectra of highly absorbing samples for re-absorption and inner filter effects.
Forest, Gallas, Hecht, Hill, Jennifer Riesz, Joel Gilmore, Kollias, Lakowicz, McGuiness, Menon, Meredith, Mosca, Nofsinger, Paul Meredith, Prota, Stark, Taylor, Teuchner, Vitkin, Williams   +19 more
core   +1 more source

A fixed combination of probiotics and herbal extracts attenuates intestinal barrier dysfunction from inflammatory stress in an in vitro model using Caco-2 cells. [PDF]

, 2019
Background: Inflammatory Bowel Diseases (IBD), are considered a growing global disease, with about ten million people being affected worldwide. Maintenance of intestinal barrier integrity is crucial for preventing IBD onset and exacerbations. Some recent
Biagi, M, Borgonetti, V, Carnevali, I, Catanzaro, D, Cocetta, V, Giron, MC, Governa, P, Montopoli, M, Ragazzi, E   +8 more
core   +1 more source

Harnessing Non‐Covalent Protein–Protein Interaction Domains for Production of Biocatalytic Materials Systems

Advanced Functional Materials, EarlyView.
Non‐covalent protein–protein interactions mediated by SH3, PDZ, or GBD domains enable the self‐assembly of stable and biocatalytically active hydrogel materials. These soft materials can be processed into monodisperse foams that, once dried, exhibit enhanced mechanical stability and activity and are easily integrated into microstructured flow ...
Julian S. Hertel, Maria Alessandra Martini, Marius Stoeckle, Annika J. Weber, Kersten S. Rabe, Christof M. Niemeyer   +5 more
wiley   +1 more source

Fluorescein as a diagnostic marker of bladder ruptures: an experimental study on rabbit model

Journal of Veterinary Research, 2016
Introduction: The aim of this study was to investigate fluorescein use in the diagnosis of bladder ruptures in rabbits as an experimental model.
Aksoy Özgür, Kurt Başak, Ermutlu Celal Şahin, Çeçen Kürşat, Yayla Sadık, Ekinci Metin, Özaydin İsa, Ünlüer Süleyman Erdinç   +7 more
doaj   +1 more source

Lensless Miniature Portable Fluorometer for Measurement of Chlorophyll and CDOM in Water Using Fluorescence Contact Imaging

IEEE Photonics Journal, 2014
We report on the design, fabrication, and verification of a proof-of-concept miniature fluorometer, which is designed to measure chlorophyll and colored dissolved organic matter (CDOM) concentration in aquatic environment.
Lior Blockstein, Orly Yadid-Pecht
doaj   +1 more source