Determination of fluorescein and fluorescein monoglucuronide excreted in urine.
A simple method for the fluorometric determination of fluorescein and fluorescein monoglucuronide in urine was developed. Free fluorescein was determined by measuring its native fluorescence at 494 nm excitation and 516 nm emission. The conjugate was determined as fluorescein after hydrolysis at 37°for 20 min with β-glucuronidase.
Hiroshi Nakamura+2 more
openaire +4 more sources
Quantitative Untersuchungen über den Speicherungsmechanismus von Rhodamin B, Eosin und Neutralrot in Hefezellen [PDF]
Die quantitative Messung der Farbstoffspeicherung von Modellsubstanzen und Hefezellen in Abhängigkeit von der CH zeigen bei den drei untersuchten Farbstoffen Rhodamin B, Eosin und Neutralrot, daß die elektrische Ladung von Adsorbens und Adsorptiv eine ...
Kölbel, Hermann
core +1 more source
Bifunctionalised long-wavelength fluorescent probes for biological applications [PDF]
The synthesis of benzo[a]phenoxazinium chlorides which are bifunctionalised in position 2 with 4-ethoxy-4-oxobutoxyl, 3-hydroxypropoxyl or 3-chloropropoxyl groups, and in position 9 with the (aminopropyl)amino group, was efficiently performed.
Firmino, A. D. G.+1 more
core +1 more source
Thermal Conductivity of Crumb Rubber as Partial Sand Replacement and Recycled Aggregates as Partial Coarse Aggregate Replacement in Concrete [PDF]
Disposal of waste tire rubber has become a major environmental issue worldwide and is increasing day by day, especially in Malaysia where carbon emission is among the highest in the world.
Mohd Zuki, Sharifah Salwa+3 more
core
Vesicular Zinc Promotes Presynaptic and Inhibits Postsynaptic Long-Term Potentiation of Mossy Fiber-CA3 Synapse [PDF]
The presence of zinc in glutamatergic synaptic vesicles of excitatory neurons of mammalian cerebral cortex suggests that zinc might regulate plasticity of synapses formed by these neurons.
Pan, Enhui+7 more
core +1 more source
Bioorthogonal Swarming: In Situ Generation of Dendrimers [PDF]
With the aid of bioorthogonal chemistry, we demonstrate the fabrication of synthetic dendrimers in situ around living cells. Using tetrazine dienophile and aminooxyl/hydrazide aldehyde chemistries, the density of functional groups on the dendrimers ...
Baibek, Assel+9 more
core +4 more sources
A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses [PDF]
The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS).
Abbink+60 more
core +2 more sources
QZ1 and QZ2: rapid, reversible quinoline-derivatized fluoresceins for sensing biological Zn(II).
QZ1, 2-[2-chloro-6-hydroxy-3-oxo-5-(quinolin-8-ylaminomethyl)-3H-xanthen-9-yl]benzoic acid, and QZ2, 2-[6-hydroxy-3-oxo-4,5-bis-(quinolin-8-ylaminomethyl)-3H-xanthen-9-yl]benzoic acid, two fluorescein-based dyes derivatized with 8-aminoquinoline, have ...
Elizabeth M. Nolan+5 more
semanticscholar +1 more source
Inhibition of phospho-MurNAc-pentapeptide translocase (MraY) by nucleoside natural product antibiotics, bacteriophage
This review covers recent developments in the inhibition of translocase MraY and related phospho-GlcNAc transferases WecA and TagO, and insight into the inhibition and catalytic mechanism of this class of integral membrane proteins from the structure of ...
Bugg, Tim+3 more
core +1 more source
Characterization of Fluorescent Proteins with Intramolecular Photostabilization* [PDF]
Genetically encodable fluorescent proteins have revolutionized biological imaging in vivo and in vitro. Despite their importance, their photophysical properties, i.
Cordes, Thorben+7 more
core +1 more source